{"doi":"10.1016/j.str.2025.07.006","title":"Antibody-like recognition of a γδ T cell receptor toward a foreign antigen","abstract":"The antigen recognition principles of B cells and αβ T cells have been well described compared to those of the γδ T cell. By way of their specificity conferring receptor (γδTCR), γδ T cells can directly bind proteinaceous antigens. A known γδ T cell and B cell model antigen is phycoerythrin (PE), a light harvesting protein from rhodophytes and cyanobacteria. Here we probed human γδTCR reactivity to PE, in which a Vδ1Vγ5 TCR bound directly to induce proximal signaling and cellular activation. We determined the cryoelectron microscopy (cryo-EM) structure of the γδTCR-phycoerythrin immune complex. We then determined the cryo-EM structures of an antibody fragment and an αβTCR bound to PE. This revealed convergent use of apical aromatic residues to mediate contacts with a common PE epitope. Comparative analyses of the γδTCR revealed multiple antibody-like characteristics, including an enrichment of apical aromatic residues. Our findings reveal further distinct facets of antigen recognition by the γδTCR.","journal":"Structure","year":2025,"id":541934,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9575,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":347611,"name":"Hariprasad Venugopal","orcid":"0000-0001-5230-2973","position":1,"is_corresponding":false},{"id":1257582,"name":"Michael T. Rice","orcid":"0000-0002-2693-0252","position":2,"is_corresponding":false},{"id":869994,"name":"Sachith D. Gunasinghe","orcid":"0000-0003-3794-3005","position":3,"is_corresponding":false},{"id":1225973,"name":"Chhon Ling Sok","orcid":"0009-0001-0705-3354","position":4,"is_corresponding":false},{"id":675821,"name":"Nicholas A. Gherardin","orcid":"0000-0003-4690-2571","position":5,"is_corresponding":false},{"id":675823,"name":"Catarina F. Almeida","orcid":"0000-0002-3393-4411","position":6,"is_corresponding":false},{"id":252300,"name":"Ildiko Van Rhijn","orcid":"0000-0002-1446-5701","position":7,"is_corresponding":false},{"id":312635,"name":"D. Branch Moody","orcid":"0000-0003-2306-3058","position":8,"is_corresponding":false},{"id":675832,"name":"Dale I. Godfrey","orcid":"0000-0002-3009-5472","position":9,"is_corresponding":false},{"id":256186,"name":"Jamie Rossjohn","orcid":"0000-0002-2020-7522","position":10,"is_corresponding":false},{"id":312632,"name":"Benjamin S. Gully","orcid":"0000-0002-8197-0871","position":11,"is_corresponding":false},{"id":1430645,"name":"Liam Rashleigh","orcid":"0009-0008-7271-229X","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-19T02:52:51.593043Z","pmid":"40744007","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}