{"doi":"10.1016/j.stemcr.2024.09.004","title":"Interleukin-1β induces trained innate immunity in human hematopoietic progenitor cells in vitro","abstract":"Innate immune cells can develop a long-lasting hyperresponsive phenotype, termed trained immunity, mediated by epigenetic and metabolic reprogramming. In mice, exposure to Bacille Calmette-Guérin (BCG), β-glucan, or Western diet induces trained immunity by reprogramming hematopoietic progenitor cells (HPCs), through interleukin-1β (IL-1β) signaling in the bone marrow (BM). We investigated whether IL-1β induces trained immunity in primary human BM-derived HPCs in vitro. We exposed human BM-derived HPCs to IL-1β for 4 h. HPCs were expanded and differentiated into monocytes followed by functional and transcriptomic characterization. IL-1β-exposed HPCs showed higher granulocyte-macrophage colony-forming units. The monocyte offspring produced more tumor necrosis factor (TNF) and IL-1β after restimulation with lipopolysaccharide (LPS) and Pam3Cys and is metabolically more active. Transcriptomic analysis showed upregulation of key atherogenic and inflammatory pathways. In conclusion, brief exposure of human BM-derived HPCs to IL-1β in vitro induces a trained immunity phenotype.","journal":"Stem Cell Reports","year":2024,"id":422058,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.957,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1068852,"name":"Willemijn Hobo","orcid":"0000-0002-8206-8185","position":1,"is_corresponding":false},{"id":1215638,"name":"Diede van Ens","orcid":"0000-0001-7273-4896","position":2,"is_corresponding":false},{"id":1215639,"name":"Elise L. Kessler","orcid":"0000-0003-1974-4571","position":3,"is_corresponding":false},{"id":225197,"name":"Boris Novakovic","orcid":"0000-0002-5623-9008","position":4,"is_corresponding":false},{"id":609008,"name":"Nicolaas Schaap","orcid":"0000-0001-7696-4752","position":5,"is_corresponding":false},{"id":1215640,"name":"Wim H. C. Rijnen","orcid":"0000-0002-7383-8053","position":6,"is_corresponding":false},{"id":104383,"name":"Leo A. B. Joosten","orcid":"0000-0001-6166-9830","position":7,"is_corresponding":false},{"id":104377,"name":"Mihai G. Netea","orcid":"0000-0003-2421-6052","position":8,"is_corresponding":false},{"id":104386,"name":"Niels P. Riksen","orcid":"0000-0001-9197-8124","position":9,"is_corresponding":false},{"id":225185,"name":"Siroon Bekkering","orcid":"0000-0003-1149-466X","position":10,"is_corresponding":false},{"id":1215637,"name":"Daniela Flores-Gomez","orcid":"0000-0003-0443-0107","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-19T01:57:46.393290Z","pmid":"39515317","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}