{"doi":"10.1016/j.stemcr.2022.12.012","title":"Generation of anti-GD2 CAR macrophages from human pluripotent stem cells for cancer immunotherapies","abstract":"Macrophages armed with chimeric antigen receptors (CARs) provide a potent new option for treating solid tumors. However, genetic engineering and scalable production of somatic macrophages remains significant challenges. Here, we used CRISPR-Cas9 gene editing methods to integrate an anti-GD2 CAR into the AAVS1 locus of human pluripotent stem cells (hPSCs). We then established a serum- and feeder-free differentiation protocol for generating CAR macrophages (CAR-Ms) through arterial endothelial-to-hematopoietic transition (EHT). CAR-M produced by this method displayed a potent cytotoxic activity against GD2-expressing neuroblastoma and melanoma in vitro and neuroblastoma in vivo. This study provides a new platform for the efficient generation of off-the-shelf CAR-Ms for antitumor immunotherapy.","journal":"Stem Cell Reports","year":2023,"id":316742,"datarank":0.6814942173405006,"base_score":4.543294782270004,"endowment":4.543294782270004,"self_citation_contribution":0.6814942173405006,"citation_network_contribution":0.0,"self_endowment_contribution":0.6814942173405006,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":93,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1021068,"name":"Sarah Webster","orcid":"0000-0002-9716-3249","position":1,"is_corresponding":false},{"id":514523,"name":"Bret Duffin","orcid":"0000-0002-0103-0610","position":2,"is_corresponding":false},{"id":109555,"name":"Matthew N. Bernstein","orcid":"0000-0002-1810-5252","position":3,"is_corresponding":false},{"id":513710,"name":"John Steill","orcid":"0000-0003-2994-3400","position":4,"is_corresponding":false},{"id":109574,"name":"Scott Swanson","orcid":"0000-0002-3314-0515","position":5,"is_corresponding":false},{"id":277445,"name":"Matthew H. Forsberg","orcid":"0000-0002-0514-6354","position":6,"is_corresponding":false},{"id":514524,"name":"Jennifer M. Bolin","orcid":"0000-0002-2502-6982","position":7,"is_corresponding":false},{"id":659975,"name":"Matthew E. Brown","orcid":"0000-0003-1836-0218","position":8,"is_corresponding":false},{"id":380629,"name":"Aditi Majumder","orcid":"0000-0001-7436-856X","position":9,"is_corresponding":false},{"id":277448,"name":"Christian M. Capitini","orcid":"0000-0002-2276-6731","position":10,"is_corresponding":false},{"id":3794,"name":"Ron Stewart","orcid":"0000-0002-9041-1828","position":11,"is_corresponding":false},{"id":443986,"name":"James A. Thomson","orcid":"0000-0003-2970-942X","position":12,"is_corresponding":false},{"id":364076,"name":"Igor I. Slukvin","orcid":"0000-0001-9693-6927","position":13,"is_corresponding":false},{"id":956838,"name":"Jue Zhang","orcid":"0000-0003-0440-1357","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:06:44.698773Z","pmid":"36638788","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}