{"doi":"10.1016/j.stemcr.2022.11.004","title":"Stress-induced reversible cell-cycle arrest requires PRC2/PRC1-mediated control of mitophagy in Drosophila germline stem cells and human iPSCs","abstract":"Following acute genotoxic stress, both normal and tumorous stem cells can undergo cell-cycle arrest to avoid apoptosis and later re-enter the cell cycle to regenerate daughter cells. However, the mechanism of protective, reversible proliferative arrest, \"quiescence,\" remains unresolved. Here, we show that mitophagy is a prerequisite for reversible quiescence in both irradiated Drosophila germline stem cells (GSCs) and human induced pluripotent stem cells (hiPSCs). In GSCs, mitofission (Drp1) or mitophagy (Pink1/Parkin) genes are essential to enter quiescence, whereas mitochondrial biogenesis (PGC1α) or fusion (Mfn2) genes are crucial for exiting quiescence. Furthermore, mitophagy-dependent quiescence lies downstream of mTOR- and PRC2-mediated repression and relies on the mitochondrial pool of cyclin E. Mitophagy-dependent reduction of cyclin E in GSCs and in hiPSCs during mTOR inhibition prevents the usual G1/S transition, pushing the cells toward reversible quiescence (G0). This alternative method of G1/S control may present new opportunities for therapeutic purposes.","journal":"Stem Cell Reports","year":2022,"id":266256,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9516,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":263379,"name":"Abdiasis M. Hussein","orcid":"0000-0001-8060-8988","position":1,"is_corresponding":false},{"id":783518,"name":"Riya Keshri","orcid":"0000-0001-7708-6402","position":2,"is_corresponding":false},{"id":395821,"name":"Julien Roy Ishibashi","orcid":"0000-0002-7273-8034","position":3,"is_corresponding":false},{"id":784144,"name":"Tung Ching Chan","orcid":null,"position":4,"is_corresponding":false},{"id":925936,"name":"Bich N. Nguyen","orcid":"0000-0001-8140-2236","position":5,"is_corresponding":false},{"id":730020,"name":"Shuozhi Liu","orcid":"0000-0002-9067-1195","position":6,"is_corresponding":false},{"id":784143,"name":"Daniel Kennedy Brewer","orcid":null,"position":7,"is_corresponding":false},{"id":926390,"name":"Stuart Harper","orcid":null,"position":8,"is_corresponding":false},{"id":587997,"name":"Scott K. Lyons","orcid":"0000-0003-0139-6054","position":9,"is_corresponding":false},{"id":926391,"name":"Ben Garver","orcid":null,"position":10,"is_corresponding":false},{"id":926392,"name":"Jimmy Dang","orcid":null,"position":11,"is_corresponding":false},{"id":886594,"name":"Nanditaa Balachandar","orcid":null,"position":12,"is_corresponding":false},{"id":926393,"name":"Samriddhi Jhajharia","orcid":null,"position":13,"is_corresponding":false},{"id":784145,"name":"Debra del Castillo","orcid":null,"position":14,"is_corresponding":false},{"id":263380,"name":"Julie Mathieu","orcid":"0000-0002-3826-3549","position":15,"is_corresponding":false},{"id":263388,"name":"Hannele Ruohola‐Baker","orcid":"0000-0002-5588-4531","position":16,"is_corresponding":false},{"id":396635,"name":"Tommy Henry Taslim","orcid":null,"position":0,"is_corresponding":true}],"reference_count":93,"raw_metadata":null,"created_at":"2026-07-19T00:26:54.081997Z","pmid":"36493777","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}