{"doi":"10.1016/j.stemcr.2022.04.013","title":"Derivation of functional trophoblast stem cells from primed human pluripotent stem cells","abstract":"Trophoblast stem cells (TSCs) have recently been derived from human embryos and early-first-trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a bone morphogetic protein 4 (BMP4)-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSCs) into functional trophoblasts; however, those trophoblasts could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to newly developed TSC medium, to derive bona fide TSCs. We show that these cells resemble placenta- and naive hPSC-derived TSCs, based on their transcriptome as well as their in vitro and in vivo differentiation potential. We conclude that primed hPSCs can be used to generate functional TSCs through a simple protocol, which can be applied to a widely available set of existing hPSCs, including induced pluripotent stem cells, derived from patients with known birth outcomes.","journal":"Stem Cell Reports","year":2022,"id":236225,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":71,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9538,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":667811,"name":"Robert Morey","orcid":"0000-0001-8480-4564","position":1,"is_corresponding":false},{"id":304372,"name":"Tony Bui","orcid":"0000-0001-9744-3120","position":2,"is_corresponding":false},{"id":684059,"name":"Daniela F. Requena","orcid":null,"position":3,"is_corresponding":false},{"id":856082,"name":"Virginia Chu Cheung","orcid":"0000-0003-2617-823X","position":4,"is_corresponding":false},{"id":683103,"name":"Sampada Kallol","orcid":"0000-0003-3327-4852","position":5,"is_corresponding":false},{"id":856083,"name":"Ryan Kittle","orcid":"0009-0007-1147-5190","position":6,"is_corresponding":false},{"id":856084,"name":"Madeline G. Jackson","orcid":"0009-0009-0253-646X","position":7,"is_corresponding":false},{"id":295432,"name":"Omar Farah","orcid":"0000-0003-4446-5478","position":8,"is_corresponding":false},{"id":856085,"name":"Jennifer N. Chousal","orcid":"0000-0002-8725-6142","position":9,"is_corresponding":false},{"id":347108,"name":"Morgan Meads","orcid":"0000-0002-5970-7356","position":10,"is_corresponding":false},{"id":335161,"name":"Donald Pizzo","orcid":"0000-0002-8022-3604","position":11,"is_corresponding":false},{"id":304371,"name":"Mariko Horii","orcid":"0000-0002-4996-6792","position":12,"is_corresponding":false},{"id":12597,"name":"Kathleen M. Fisch","orcid":"0000-0002-0117-7444","position":13,"is_corresponding":false},{"id":295434,"name":"Mana M. Parast","orcid":"0000-0001-5963-2246","position":14,"is_corresponding":false},{"id":683105,"name":"Francesca Soncin","orcid":"0000-0002-7423-9167","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T00:21:58.728756Z","pmid":"35594858","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}