{"doi":"10.1016/j.stemcr.2017.09.024","title":"Escape from Pluripotency via Inhibition of TGF-β/BMP and Activation of Wnt Signaling Accelerates Differentiation and Aging in hPSC Progeny Cells","abstract":null,"journal":"Stem Cell Reports","year":2017,"id":662044,"datarank":0.6456097639806255,"base_score":4.304065093204169,"endowment":4.304065093204169,"self_citation_contribution":0.6456097639806255,"citation_network_contribution":0.0,"self_endowment_contribution":0.6456097639806255,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":73,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":852436,"name":"Takuya Matsumoto","orcid":"0000-0002-8950-6598","position":1,"is_corresponding":false},{"id":1728339,"name":"Fumihiko Kisa","orcid":null,"position":2,"is_corresponding":false},{"id":17418,"name":"Nobutaka Hattori","orcid":"0000-0002-2034-2556","position":3,"is_corresponding":false},{"id":269746,"name":"Hideyuki Okano","orcid":"0000-0001-7482-5935","position":4,"is_corresponding":false},{"id":273561,"name":"Wado Akamatsu","orcid":"0009-0003-4947-1428","position":5,"is_corresponding":false},{"id":1728338,"name":"Koki Fujimori","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Escape from Pluripotency via Inhibition of TGF-β/BMP and Activation of Wnt Signaling Accelerates Differentiation and Aging in hPSC Progeny Cells","abstract":"Human pluripotent stem cells (hPSCs) represent a potentially valuable cell source for applications in cell replacement therapy, drug development, and disease modeling. For all these uses, it is necessary to develop reproducible and robust protocols for differentiation into desired cell types. However, differentiation protocols remain unstable and inefficient, which makes minimizing the differentiation variance among hPSC lines and obtaining purified terminally differentiated cells extremely time consuming. Here, we report a simple treatment with three small molecules-SB431542, dorsomorphine, and CHIR99021-that enhanced hPSC differentiation into three germ layers with a chemically transitional embryoid-body-like state (CTraS). Induction of CTraS reduced the innate differentiation propensities of hPSCs (even unfavorably differentiated hPSCs) and shifted their differentiation into terminally differentiated cells, particularly neurons. In addition, CTraS induction accelerated in vitro pathological expression concurrently with neural maturation. Thus, CTraS can promote the latent potential of hPSCs for differentiation and potentially expand the utility and applicability of hPSCs.","is_dataset_classified":null,"base_score":4.304065093204169,"endowment":4.304065093204169,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29107593","pmcid":"PMC5831048","openalex_id":"https://openalex.org/W2765664591","authors":[],"funders":[{"funder_name":"Japan Society for the Promotion of Science","grant_id":"JP16J06437","title":null},{"funder_name":"Japan Society for the Promotion of Science","grant_id":"JP16K09677","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"15bk0104027h0003","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"16bk0104016h0004","title":null},{"funder_name":"Japan Science and Technology Agency","grant_id":"15bm0609003h0004","title":null},{"funder_name":"Japan Science and Technology Agency","grant_id":"12033011","title":null},{"funder_name":"Japan Science and Technology Agency","grant_id":"16bm0609003h0005","title":null},{"funder_name":"New Energy and Industrial Technology Development Organization","grant_id":"17be0204436h0004","title":null},{"funder_name":"MEXT","grant_id":"","title":null},{"funder_name":"Keio University Grant-in-Aid for the Encouragement of Young Medical Scientists","grant_id":"","title":null},{"funder_name":"Keio University Doctorate Student Grant-in-Aid Program","grant_id":"","title":null}],"total_grants":11,"fwci":3.0452,"citation_percentile":0.92571844,"influential_citations":0,"citation_trend":[{"year":2018,"count":7},{"year":2019,"count":12},{"year":2020,"count":11},{"year":2021,"count":16},{"year":2022,"count":3},{"year":2023,"count":7},{"year":2024,"count":5},{"year":2025,"count":8},{"year":2026,"count":4}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"http://www.cell.com/article/S2213671117304319/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S2213671117304319/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2213671117304319?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2213671117304319?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.stemcr.2017.09.024","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29107593","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5831048","host_type":"repository"},{"url":"https://doaj.org/article/b7b0d51b34784cfa84971d6f6b0a89ec","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5831048","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5831048?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Pluripotent Stem Cells Research","CRISPR and Genetic Engineering","Tissue Engineering and Regenerative Medicine"],"mesh_terms":["Human Embryonic Stem Cells","Benzamides","Cell Differentiation","Cell Line","Cells, Cultured","Dioxoles","Humans","Neurons","Pyrazoles","Pyridines","Pyrimidines","Transforming Growth Factor beta","Cellular Senescence","Receptors, Transforming Growth Factor beta","Bone Morphogenetic Proteins","Protein Kinase Inhibitors","Embryoid Bodies","Wnt Signaling Pathway"],"keywords":["Induced pluripotent stem cell","Biology","Cellular differentiation","Wnt signaling pathway","Embryoid body","Cell biology","Directed differentiation","Germ layer","Regenerative medicine","Embryonic stem cell","Stem cell","Signal transduction","Genetics","Gene","Aging","Differentiation","Pluripotency","Disease model","Induced Pluripotent Stem Cells","Stem Cell Differentiation","Stem Cell Biotechnology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T12:19:22.458780Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}