{"doi":"10.1016/j.stem.2023.07.014","title":"Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells","abstract":"Life-long reconstitution of a tissue's resident stem cell compartment with engrafted cells has the potential to durably replenish organ function. Here, we demonstrate the engraftment of the airway epithelial stem cell compartment via intra-airway transplantation of mouse or human primary and pluripotent stem cell (PSC)-derived airway basal cells (BCs). Murine primary or PSC-derived BCs transplanted into polidocanol-injured syngeneic recipients give rise for at least two years to progeny that stably display the morphologic, molecular, and functional phenotypes of airway epithelia. The engrafted basal-like cells retain extensive self-renewal potential, evident by the capacity to reconstitute the tracheal epithelium through seven generations of secondary transplantation. Using the same approach, human primary or PSC-derived BCs transplanted into NOD scid gamma (NSG) recipient mice similarly display multilineage airway epithelial differentiation in vivo. Our results may provide a step toward potential future syngeneic cell-based therapy for patients with diseases resulting from airway epithelial cell damage or dysfunction.","journal":"Cell stem cell","year":2023,"id":318542,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":64,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":328225,"name":"Bibek R. Thapa","orcid":"0000-0001-5254-0808","position":1,"is_corresponding":false},{"id":868130,"name":"Jake A. Le Suer","orcid":null,"position":2,"is_corresponding":false},{"id":266488,"name":"Andrew Tilston-Lünel","orcid":"0000-0002-5582-6884","position":3,"is_corresponding":false},{"id":228728,"name":"Michael J. Herriges","orcid":"0000-0002-7842-9515","position":4,"is_corresponding":false},{"id":240008,"name":"Andrew Berical","orcid":"0000-0002-0818-0259","position":5,"is_corresponding":false},{"id":234395,"name":"Mary Lou Beermann","orcid":null,"position":6,"is_corresponding":false},{"id":880828,"name":"Feiya Wang","orcid":"0000-0002-7507-6275","position":7,"is_corresponding":false},{"id":260367,"name":"Pushpinder Bawa","orcid":"0000-0002-8370-3638","position":8,"is_corresponding":false},{"id":1025851,"name":"Anat Kohn","orcid":"0000-0002-8501-6006","position":9,"is_corresponding":false},{"id":228729,"name":"Alexandra B. Ysasi","orcid":"0000-0002-2932-870X","position":10,"is_corresponding":false},{"id":1025852,"name":"Hirofumi Kiyokawa","orcid":"0000-0001-8207-9688","position":11,"is_corresponding":false},{"id":240009,"name":"Taylor Matte","orcid":"0000-0002-7796-7485","position":12,"is_corresponding":false},{"id":72632,"name":"Scott H. Randell","orcid":"0000-0001-5351-2841","position":13,"is_corresponding":false},{"id":266489,"name":"Xaralabos Varelas","orcid":"0000-0002-2882-4541","position":14,"is_corresponding":false},{"id":232299,"name":"Finn Hawkins","orcid":"0000-0002-2246-6448","position":15,"is_corresponding":false},{"id":228731,"name":"Darrell N. Kotton","orcid":"0000-0002-9604-8476","position":16,"is_corresponding":false},{"id":987306,"name":"Liang Ma","orcid":"0000-0003-2938-0716","position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-19T01:07:01.300821Z","pmid":"37625411","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}