{"doi":"10.1016/j.semcdb.2012.05.006","title":"Quantitative phosphoproteomics to characterize signaling networks","abstract":null,"journal":"Seminars in Cell & Developmental Biology","year":2012,"id":594520,"datarank":0.62147020895873,"base_score":4.143134726391533,"endowment":4.143134726391533,"self_citation_contribution":0.62147020895873,"citation_network_contribution":0.0,"self_endowment_contribution":0.62147020895873,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":62,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":864564,"name":"Blagoy Blagoev","orcid":"0000-0002-3596-0066","position":1,"is_corresponding":false},{"id":1521978,"name":"Kristoffer T.G. Rigbolt","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Quantitative phosphoproteomics to characterize signaling networks","abstract":"Reversible protein phosphorylation is involved in the regulation of most, if not all, major cellular processes via dynamic signal transduction pathways. During the last decade quantitative phosphoproteomics have evolved from a highly specialized area to a powerful and versatile platform for analyzing protein phosphorylation at a system-wide scale and has become the intuitive strategy for comprehensive characterization of signaling networks. Contemporary phosphoproteomics use highly optimized procedures for sample preparation, mass spectrometry and data analysis algorithms to identify and quantify thousands of phosphorylations, thus providing extensive overviews of the cellular signaling networks. As a result of these developments quantitative phosphoproteomics have been applied to study processes as diverse as immunology, stem cell biology and DNA damage. Here we review the developments in phosphoproteomics technology that have facilitated the application of phosphoproteomics to signaling networks and introduce examples of recent system-wide applications of quantitative phosphoproteomics. Despite the great advances in phosphoproteomics technology there are still several outstanding issues and we provide here our outlook on the current limitations and challenges in the field.","is_dataset_classified":null,"base_score":4.143134726391533,"endowment":4.143134726391533,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22677334","pmcid":null,"openalex_id":"https://openalex.org/W2021238335","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"201648","title":"PROteomics SPECification in Time and Space"}],"total_grants":1,"fwci":3.782,"citation_percentile":0.9424649,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":9},{"year":2014,"count":9},{"year":2015,"count":7},{"year":2016,"count":2},{"year":2017,"count":6},{"year":2018,"count":4},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":4},{"year":2022,"count":4},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":3}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"https://zenodo.org/record/895950","host_type":"repository"},{"url":"https://zenodo.org/record/895950","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S1084952112001073?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1084952112001073?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.semcdb.2012.05.006","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22677334","host_type":"repository"},{"url":"https://portal.findresearcher.sdu.dk/da/publications/d6c15bc9-823a-46ca-9b2b-c671f0b07832","host_type":"repository"},{"url":"https://zenodo.org/record/895950/files/article.pdf","host_type":""},{"url":"http://dx.doi.org/10.1016/j.semcdb.2012.05.006","host_type":""},{"url":"https://dx.doi.org/10.1016/j.semcdb.2012.05.006","host_type":""},{"url":"https://zenodo.org/records/895950","host_type":""}],"fields_of_study":["Advanced Proteomics Techniques and Applications","Mass Spectrometry Techniques and Applications","Advanced Biosensing Techniques and Applications","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Animals","Humans","Phosphoproteins","Phosphorylation","Mass Spectrometry","Signal Transduction","Proteomics"],"keywords":["Phosphoproteomics","Computational biology","Proteomics","Biology","Systems biology","Quantitative proteomics","Phosphorylation","Computer science","Protein phosphorylation","Cell biology","Protein kinase A","Genetics","Animals","Humans","Phosphoproteins","Mass Spectrometry","Signal Transduction"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T14:48:16.746364Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}