{"doi":"10.1016/j.sbi.2020.10.009","title":"Building ubiquitination machineries: E3 ligase multi-subunit assembly and substrate targeting by PROTACs and molecular glues","abstract":null,"journal":"Current Opinion in Structural Biology","year":2021,"id":676486,"datarank":2.2514682999764193,"base_score":4.174387269895637,"endowment":4.174387269895637,"self_citation_contribution":0.6261580904843456,"citation_network_contribution":1.6253102094920737,"self_endowment_contribution":0.6261580904843456,"citer_contribution":1.6253102094920737,"corpus_percentile":null,"corpus_rank":null,"citation_count":64,"citer_count":55,"citers_with_citation_signal":50,"citers_with_endowment":50,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":261818,"name":"Alessio Ciulli","orcid":"0000-0002-8654-1670","position":1,"is_corresponding":false},{"id":1767540,"name":"Sarath Ramachandran","orcid":"0000-0002-0922-9448","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Building ubiquitination machineries: E3 ligase multi-subunit assembly and substrate targeting by PROTACs and molecular glues","abstract":"E3 ubiquitin ligase machineries are emerging as attractive therapeutic targets because they confer specificity to substrate ubiquitination and can be hijacked for targeted protein degradation. In this review, we bring to focus our current structural understanding of E3 ligase complexes, in particular the multi-subunit cullin RING ligases, and modulation thereof by small-molecule glues and PROTAC degraders. We highlight recent advances in elucidating the modular assembly of E3 ligase machineries, their diverse substrate and degron recognition mechanisms, and how these structural features impact on ligase function. We then outline the emergence of structures of E3 ligases bound to neo-substrates and degrader molecules, and highlight the importance of studying such ternary complexes for structure-based degrader design.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26207759","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"European Commission","grant_id":"311460","title":"Probing Druggability of Multisubunit Complexes: \nE3 Cullin RING Ligases"},{"funder_name":"European Commission","grant_id":"875510","title":"EUbOPEN: Enabling and Unlocking biology in the OPEN"}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"Elsevier TDM","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S0959440X20301755","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0959440X20301755?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0959440X20301755?httpAccept=text/plain","host_type":"publisher"},{"url":"https://discovery.dundee.ac.uk/ws/files/54885780/1_s2.0_S0959440X20301755_main.pdf","host_type":"repository"},{"url":"https://doi.org/10.1016/j.sbi.2020.10.009","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/33271439","host_type":""},{"url":"https://dx.doi.org/10.1016/j.sbi.2020.10.009","host_type":""},{"url":"http://dx.doi.org/10.1016/j.sbi.2020.10.009","host_type":""},{"url":"https://doi.org/https://doi.org/10.1016/j.sbi.2020.10.009","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["570","name=Structural Biology","/dk/atira/pure/subjectarea/asjc/1300/1312","Ubiquitin-Protein Ligases","Proteolysis","Ubiquitination","name=Molecular Biology","/dk/atira/pure/subjectarea/asjc/1300/1315"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T02:39:11.720331Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}