{"doi":"10.1016/j.sbi.2016.06.001","title":"Cryo-electron microscopy for structure analyses of membrane proteins in the lipid bilayer","abstract":null,"journal":"Current Opinion in Structural Biology","year":2016,"id":606211,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1556028,"name":"Yoshinori Fujiyoshi","orcid":null,"position":1,"is_corresponding":false},{"id":1494693,"name":"Kazuhiro Abe","orcid":"0000-0001-6197-4659","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cryo-electron microscopy for structure analyses of membrane proteins in the lipid bilayer","abstract":"Asymmetric distributions of materials, such as ions, solutes, chemicals, and information across the bio-membrane, which are generated by membrane proteins, are crucial hallmarks of all living organisms. Since the appropriate asymmetric distribution depends on the precise function of membrane proteins in the lipid bilayer, structural and functional analyses of the membrane-embedded forms of these proteins are crucial toward elucidating their mechanisms. In this review, we provide remarkable examples of structural analyses of membrane proteins in lipid bilayers based on electron crystallography of two-dimensional crystals. We also discuss the recent development of single-particle cryo-electron microscopy and propose a conceptual strategy for structural analysis of membrane proteins in the lipid bilayer.","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27309495","pmcid":null,"openalex_id":"https://openalex.org/W2422656563","authors":[],"funders":[{"funder_name":"Grants-in-Aid for Young Scientists","grant_id":"","title":null},{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"","title":null},{"funder_name":"Japan New Energy and Industrial Technology Development Organization","grant_id":"","title":null},{"funder_name":"Core Research for Evolutional Science and Technology from JST","grant_id":"","title":null},{"funder_name":"MEXT","grant_id":"","title":null}],"total_grants":5,"fwci":0.962,"citation_percentile":0.76563227,"influential_citations":0,"citation_trend":[{"year":2016,"count":2},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"http://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0959440X16300586?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0959440X16300586?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.sbi.2016.06.001","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27309495","host_type":"repository"}],"fields_of_study":["Lipid Membrane Structure and Behavior","Photosynthetic Processes and Mechanisms","Spectroscopy and Quantum Chemical Studies"],"mesh_terms":["Lipid Bilayers","Membrane Proteins","Crystallography, X-Ray","Cryoelectron Microscopy","Aquaporins"],"keywords":["Lipid bilayer","Membrane protein","Protein–lipid interaction","Peripheral membrane protein","Bilayer","Membrane","Biophysics","Electron microscope","Chemistry","Membrane fluidity","Structural biology","Electron crystallography","Cryo-electron microscopy","Elasticity of cell membranes","Membrane biophysics","Lipid bilayer phase behavior","Integral membrane protein","Biology","Biochemistry","Electron diffraction","Physics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T04:07:02.552321Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}