{"doi":"10.1016/j.rpth.2025.102680","title":"Transcription factor RUNX1 regulates coagulation factor XIII-A (F13A1): decreased platelet-megakaryocyte F13A1 expression and clot contraction in RUNX1 haplodeficiency","abstract":"Background Germline RUNX1 haplodeficiency (RHD) is associated with thrombocytopenia, platelet dysfunction, and predisposition to myeloid malignancies. Platelet expression profiling of an RHD patient showed decreased F13A1 , encoding for the A subunit of factor (F)XIII , a transglutaminase that cross-links fibrin and induces clot stabilization. FXIII-A is synthesized by hematopoietic cells, megakaryocytes, and monocytes. Objectives To understand RUNX1 regulation of F13A1 expression in platelets/megakaryocytes and the mechanisms and consequences of decreased F13A1 in RHD. Methods We performed studies in platelets, human erythroleukemia (HEL) cells, and human CD34+ cell-derived megakaryocytes including on clot contraction in cells following small inhibitor RNA knockdown (KD) of RUNX1 or F13A1. Results Platelet F13A1 mRNA and protein were decreased in our index patient and in 2 siblings from an unrelated family with RHD. Platelet-driven clot contraction was decreased in the patient and affected daughter. Promoter studies in HEL cells showed that RUNX1 regulates F13A1 transcription ; RUNX1 overexpression increased, and small inhibitor RNA RUNX1 KD reduced F13A1 promoter activity and protein. Following RUNX1 or F13A1 KD, clot contraction by HEL cells was decreased, as were FXIII-A surface expression, myosin light chain phosphorylation, and PAC1 antibody binding upon activation. F13A1 expression and clot contraction were impaired in RUNX1 downregulation in human megakaryocytes. Conclusion RUNX1 regulates platelet-megakaryocyte F13A1 expression, which is decreased in RHD, reflecting regulation of a coagulation protein by a hematopoietic transcription factor. Platelet and megakaryocyte clot contraction is decreased in RHD, related to multiple impaired mechanisms including F13A1 expression , myosin phosphorylation, and α IIb β 3 activation.","journal":"Research and Practice in Thrombosis and Haemostasis","year":2025,"id":560541,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1320771,"name":"Natthapol Songdej","orcid":"0000-0001-6545-7931","position":1,"is_corresponding":false},{"id":1282503,"name":"Liying Guan","orcid":"0000-0002-7470-564X","position":2,"is_corresponding":false},{"id":938456,"name":"Guangfen Mao","orcid":null,"position":3,"is_corresponding":false},{"id":492801,"name":"Lawrence E. Goldfinger","orcid":"0000-0003-0525-6008","position":4,"is_corresponding":false},{"id":493361,"name":"Jeremy G. T. Wurtzel","orcid":null,"position":5,"is_corresponding":false},{"id":1194119,"name":"Kiwon Lee","orcid":"0000-0003-1340-3602","position":6,"is_corresponding":false},{"id":260212,"name":"Michele P. Lambert","orcid":"0000-0003-0439-402X","position":7,"is_corresponding":false},{"id":299573,"name":"Mortimer Poncz","orcid":"0000-0001-7237-3613","position":8,"is_corresponding":false},{"id":422362,"name":"A. Koneti Rao","orcid":"0000-0002-3078-7778","position":9,"is_corresponding":false},{"id":787349,"name":"Fabiola Del Carpio‐Cano","orcid":null,"position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":null,"created_at":"2026-07-19T02:55:42.883572Z","pmid":"39995753","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}