{"doi":"10.1016/j.redox.2024.103425","title":"Dehydroascorbic acid quantification in human plasma: Simultaneous direct measurement of the ascorbic acid/dehydroascorbic acid couple by UPLC/MS-MS","abstract":"Ascorbic acid (AA, vitamin C) and dehydroascorbic acid (DHA) constitute a biological couple. No technique can accurately, independently, and simultaneously quantify both members of the couple in animal and human samples, thereby constraining advances in physiology and pathophysiology. Here we describe a new UPLC/MS/MS method to measure both compounds directly and independently in human plasma. Lower limits of quantification were 16 nM, with linear coefficients >0.99 over a 100-fold concentration range. The method was stable and reproducible with <10 % injection-to-injection variation. Use of isotopic labeled internal standards for both compounds ensured precision and accuracy. Plasma preparation required only 2 steps. In plasma samples from 14 anonymized subjects who met criteria for blood donation, mean concentrations were 6±2 μmol/L (mean ± SD) and 56 ± 14 μmol/L for DHA and AA respectively, with (DHA)/(AA + DHA) ratio of 9.8 %. This method represents a pioneering approach to measuring the AA/DHA couple in human plasma.","journal":"Redox Biology","year":2024,"id":429048,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9516,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1219184,"name":"Nicholas Munyan","orcid":null,"position":1,"is_corresponding":false},{"id":705138,"name":"Hartmut Luecke","orcid":"0000-0002-4938-0775","position":2,"is_corresponding":false},{"id":573664,"name":"Y. Wang","orcid":"0000-0001-9849-0682","position":3,"is_corresponding":false},{"id":1231696,"name":"J. Lloyd","orcid":null,"position":4,"is_corresponding":false},{"id":1218626,"name":"Kartick Patra","orcid":"0000-0002-5636-7739","position":5,"is_corresponding":false},{"id":1231697,"name":"Katherine M. Blakeslee","orcid":null,"position":6,"is_corresponding":false},{"id":376787,"name":"Ifechukwude Ebenuwa","orcid":"0000-0002-5234-1347","position":7,"is_corresponding":false},{"id":360755,"name":"Mark Levine","orcid":"0000-0003-2800-8728","position":8,"is_corresponding":false},{"id":364399,"name":"Pierre-Christian Violet","orcid":"0000-0001-9550-3660","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":null,"created_at":"2026-07-19T01:59:06.668462Z","pmid":"39591903","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}