{"doi":"10.1016/j.redox.2024.103330","title":"Intermittent ozone inhalation during house dust mite-induced sensitization primes for adverse asthma phenotype","abstract":"The ability of air pollution to induce acute exacerbation of asthma is well documented. However, the ability of ozone (O 3 ), the most reactive gaseous component of air pollution, to function as a modulator during sensitization is not well established. C57BL/6 J male mice were intranasally sensitized to house dust mite (HDM) (40 μg/kg) for 3 weeks on alternate days in parallel with once-a-week O 3 exposure (1 ppm). Mice were euthanized 24 h following the last HDM challenge. Lung lavage, histology, lung function (both forced oscillation and forced expiration-based), immune cell profiling, inflammation (pulmonary and systemic), and immunoglobulin production were assessed. Compared to HDM alone, HDM + O 3 leads to a significant increase in peribronchial inflammation (p < 0.01), perivascular inflammation (p < 0.001) and methacholine-provoked large airway hyperreactivity (p < 0.05). Serum total IgG and IgE and HDM-specific IgG1 were 3–5 times greater in HDM + O 3 co-exposure compared to PBS and O 3 -exposed groups. An increase in activated/mature lung total and monocyte-derived dendritic cells (p < 0.05) as well as T-activated, and T memory lymphocyte subset numbers (p < 0.05) were noted in the HDM + O 3 group compared to HDM alone group. Concurrent O 3 inhalation and HDM sensitization also caused significantly greater (p < 0.05) lung tissue interleukin-17 pathway gene expression and mediator levels in the serum. Redox imbalance was manifested by impaired lung antioxidant defense and increased oxidants. O 3 inhalation during allergic sensitization coalesces in generating a significantly worse T H 17 asthmatic phenotype. • Intermittent O 3 inhalation primes for aggravated asthmatic responses. • O 3 aggravates HDM-induced lung inflammation and mucin expression. • O 3 co-exposure aggravates HDM-induced mixed Th2/Th17 phenotype. • O 3 and HDM co-exposure increases the expression of DC maturation (CD80, Dectin1) and migration (CCR7) markers.","journal":"Redox Biology","year":2024,"id":447369,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":667508,"name":"Nairrita Majumder","orcid":"0000-0002-7297-7019","position":1,"is_corresponding":false},{"id":749852,"name":"Md Habibul Hasan Mazumder","orcid":"0000-0002-7807-8372","position":2,"is_corresponding":false},{"id":471316,"name":"Sara E. Lewis","orcid":"0000-0001-8119-7076","position":3,"is_corresponding":false},{"id":1265438,"name":"Olanrewaju Olapeju","orcid":null,"position":4,"is_corresponding":false},{"id":313064,"name":"Murugesan Velayutham","orcid":null,"position":5,"is_corresponding":false},{"id":868845,"name":"Md Shahrier Amin","orcid":"0000-0003-3791-508X","position":6,"is_corresponding":false},{"id":340913,"name":"Kathleen M. Brundage","orcid":null,"position":7,"is_corresponding":false},{"id":247931,"name":"Eric E. Kelley","orcid":"0000-0002-0393-1498","position":8,"is_corresponding":false},{"id":646630,"name":"Jeroen Vanoirbeek","orcid":"0000-0001-5508-3518","position":9,"is_corresponding":false},{"id":292268,"name":"Salik Hussain","orcid":"0000-0003-0339-6226","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-19T02:01:59.101070Z","pmid":"39244793","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}