{"doi":"10.1016/j.redox.2020.101504","title":"Oral high dose vitamin B12 decreases renal superoxide and post-ischemia/reperfusion injury in mice","abstract":"Renal ischemia/reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI), a potentially fatal syndrome characterized by a rapid decline in kidney function. Excess production of superoxide contributes to the injury. We hypothesized that oral administration of a high dose of vitamin B12 (B12 - cyanocobalamin), which possesses a superoxide scavenging function, would protect kidneys against IRI and provide a safe means of treatment. Following unilateral renal IR surgery, C57BL/6J wild type (WT) mice were administered B12 via drinking water at a dose of 50 mg/L. After 5 days of the treatment, plasma B12 levels increased by 1.2-1.5x, and kidney B12 levels increased by 7-8x. IRI mice treated with B12 showed near normal renal function and morphology. Further, IRI-induced changes in RNA and protein markers of inflammation, fibrosis, apoptosis, and DNA damage response (DDR) were significantly attenuated by at least 50% compared to those in untreated mice. Moreover, the presence of B12 at 0.3 μM in the culture medium of mouse proximal tubular cells subjected to 3 hr of hypoxia followed by 1 hr of reperfusion in vitro showed similar protective effects, including increased cell viability and decreased reactive oxygen species (ROS) level. We conclude that a high dose of B12 protects against perfusion injury both in vivo and in vitro without observable adverse effects in mice and suggest that B12 merits evaluation as a treatment for I/R-mediated AKI in humans.","journal":"Redox Biology","year":2020,"id":92351,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":384820,"name":"Edward Moreira Bahnson","orcid":"0000-0001-8578-0517","position":1,"is_corresponding":false},{"id":462680,"name":"Jennifer Wilder","orcid":null,"position":2,"is_corresponding":false},{"id":461843,"name":"Robin Siletzky","orcid":"0000-0002-3891-1374","position":3,"is_corresponding":false},{"id":462681,"name":"John R. Hagaman","orcid":null,"position":4,"is_corresponding":false},{"id":462682,"name":"Volker Nickekeit","orcid":null,"position":5,"is_corresponding":false},{"id":422319,"name":"Sylvia Hiller","orcid":"0000-0001-6838-1178","position":6,"is_corresponding":false},{"id":462683,"name":"Azraa Ayesha","orcid":null,"position":7,"is_corresponding":false},{"id":462684,"name":"Lanfei Feng","orcid":null,"position":8,"is_corresponding":false},{"id":461844,"name":"Jerrold S. Levine","orcid":"0000-0003-0158-6675","position":9,"is_corresponding":false},{"id":461845,"name":"Nobuyuki Takahashi","orcid":"0000-0002-9816-6185","position":10,"is_corresponding":false},{"id":412512,"name":"Nobuyo Maeda-Smithies","orcid":null,"position":11,"is_corresponding":false},{"id":461842,"name":"Feng Li","orcid":"0000-0002-9700-7844","position":0,"is_corresponding":true}],"reference_count":63,"raw_metadata":null,"created_at":"2026-07-18T22:29:54.256205Z","pmid":"32182573","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}