{"doi":"10.1016/j.psj.2025.105929","title":"Decoding the B-cell immune landscape in duck hepatitis A virus type 3 through single-cell genomics","abstract":null,"journal":"Poultry Science","year":2025,"id":607181,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1558954,"name":"Yaru Fan","orcid":null,"position":1,"is_corresponding":false},{"id":655819,"name":"Dalin He","orcid":"0000-0002-8099-146X","position":2,"is_corresponding":false},{"id":1193982,"name":"Mian Wu","orcid":"0000-0003-4233-8184","position":3,"is_corresponding":false},{"id":432916,"name":"Xinyuan Xu","orcid":"0009-0002-0122-1131","position":4,"is_corresponding":false},{"id":1558958,"name":"Xinhong Man","orcid":null,"position":5,"is_corresponding":false},{"id":1558960,"name":"Siming Zhu","orcid":null,"position":6,"is_corresponding":false},{"id":1221027,"name":"Ruiqi Shi","orcid":null,"position":7,"is_corresponding":false},{"id":1558961,"name":"Yafei Qin","orcid":"0009-0002-7244-1844","position":8,"is_corresponding":false},{"id":1558963,"name":"HongYu Ma","orcid":null,"position":9,"is_corresponding":false},{"id":738850,"name":"Qiang Feng","orcid":"0000-0002-8814-6594","position":10,"is_corresponding":false},{"id":391296,"name":"Yi Tang","orcid":"0000-0001-9066-1192","position":11,"is_corresponding":false},{"id":1558964,"name":"Youxiang Diao","orcid":"0009-0001-1871-0794","position":12,"is_corresponding":false},{"id":1558951,"name":"Saisai Zhao","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Decoding the B-cell immune landscape in duck hepatitis A virus type 3 through single-cell genomics","abstract":"Antibodies protect the organism against many diseases. How the protective antibody memory response against Duck Viral Hepatitis type 3 (DHAV-3) activates transcriptional programs, cell proliferation, and antigen-specific antibody production is unknown. To explore the mechanisms of DHAV-3 immunity and identify potential therapeutic targets, we performed single-cell RNA sequencing (scRNA-seq) and B cell receptor (BCR) library analyses to characterize the cellular landscape of splenic B-cells from mice immunized with DHAV-3. We observed significant changes in the number and proportion of B cell populations induced by DHAV-3. Through integrating single-cell gene expression profiling and BCR analysis, we have creatively identified a novel IGHV1 germline clone, IGHV1-20, which has not been previously described in the literature. Interestingly, a trend of enhanced cellular communication was observed between a novel B-cell subset, Gm37915+ B cells, and Nuggc+ B cells. Furthermore, we identified two specifically upregulated integrin subunits, ITGAL and ITGB2, in the experimental group (EG), which possibly correlated with the activation status of B cells and enhanced immune-cell interactions. Our findings not only shed light on the immunological characteristics of splenic B cells in the context of DHAV-3 immunity but also provide important advances in our understanding of targeted antibodies against this virus.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41056664","pmcid":"PMC12538067","openalex_id":"https://openalex.org/W4414691723","authors":[],"funders":[{"funder_name":"Key Technology Research and Development Program of Shandong Province","grant_id":"2022CXPT005-01-03","title":null},{"funder_name":"Key Technology Research and Development Program of Shandong Province","grant_id":"2022CXPT005-01-02","title":null},{"funder_name":"Ministry of Agriculture and Rural Affairs of the People's Republic of China","grant_id":"CARS-42-19","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2024YFF1000900","title":null},{"funder_name":"Shandong Agricultural University","grant_id":"SDAUA-2024-26","title":null},{"funder_name":"Agriculture Research System of China","grant_id":"","title":null}],"total_grants":6,"fwci":0.8934,"citation_percentile":0.79609683,"influential_citations":0,"citation_trend":[{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1016/j.psj.2025.105929","host_type":"journal"},{"url":"https://doi.org/10.1016/j.psj.2025.105929","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0032579125011691?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0032579125011691?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41056664","host_type":"repository"},{"url":"https://doaj.org/article/664a01fe30cd4422a633bab403cfd330","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12538067","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC12538067","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12538067?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Viral Infections and Immunology Research","Animal Virus Infections Studies","Viral gastroenteritis research and epidemiology","Animals","B-Lymphocytes","Single-Cell Analysis","Ducks","Hepatitis, Viral, Animal","Hepatitis Virus, Duck","Poultry Diseases","Mice","Picornaviridae Infections","Genomics"],"mesh_terms":["Animals","B-Lymphocytes","Hepatitis Virus, Duck","Ducks","Hepatitis, Viral, Animal","Picornaviridae Infections","Poultry Diseases","Genomics","Mice","Single-Cell Analysis"],"keywords":["Antibody","Immunity","Immune system","Context (archaeology)","Gene","Virus","Genome","Transcriptome","B cell","Innate immune system","B cells","B Cell Receptor","Single Cell Rna Sequencing","Dhav-3"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T05:56:12.731715Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}