{"doi":"10.1016/j.plasmid.2022.102649","title":"An easily modifiable conjugative plasmid for studying horizontal gene transfer","abstract":null,"journal":"Plasmid","year":2022,"id":675312,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1442590,"name":"Asmus Kalckar Olesen","orcid":"0000-0003-2480-6431","position":1,"is_corresponding":false},{"id":579606,"name":"Lorrie Maccario","orcid":"0000-0002-6069-6463","position":2,"is_corresponding":false},{"id":1764414,"name":"Jonas Stenløkke Madsen","orcid":null,"position":3,"is_corresponding":false},{"id":1536503,"name":"Qinqin Wang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"An easily modifiable conjugative plasmid for studying horizontal gene transfer","abstract":"Horizontal gene transfer is an important mechanism in bacterial evolution and can occur at striking frequencies when mediated by mobile genetic elements. Conjugative plasmids are mobile genetic elements that are main drivers of horizontal transfer and a major facilitator in the spread of antibiotic resistance genes. However, conjugative plasmid models that readily can be genetically modified with the aim to study horizontal transfer are not currently available. The aim of this study was to develop a conjugative plasmid model where the insertion of gene cassettes such as reporter genes (e.g., fluorescent proteins) or antibiotic resistance genes would be efficient and convenient. Here, we introduced a single attTn7 site into the conjugative broad-host-range IncP-1 plasmid pKJK5 in a non-disruptive manner. Furthermore, a version with lower transfer rate and a non-conjugative version of pKJK5-attTn7 were also constructed. The advantage of having the attTn7 sites is that genes of interest can be introduced in a single step with very high success rate using the Tn7 transposition system. In addition, larger genetic fragments can be inserted. To illustrate the efficacy of the constructed pKJK5 plasmids, they were complemented with sfGFP (a gene encoding superfolder green fluorescent protein) in addition to seven different β-lactamase genes representing the four known classes of β-lactamases.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36100085","pmcid":null,"openalex_id":"https://openalex.org/W4295136113","authors":[],"funders":[{"funder_name":"Villum Fonden","grant_id":"00028304","title":null}],"total_grants":1,"fwci":0.3524,"citation_percentile":0.45915068,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S0147619X22000336/pdf","host_type":"journal"},{"url":"https://www.sciencedirect.com/science/article/pii/S0147619X22000336/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0147619X22000336?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0147619X22000336?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.plasmid.2022.102649","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36100085","host_type":"repository"},{"url":"https://researchprofiles.ku.dk/da/publications/800eef84-5f88-43e4-adf3-1179b4ed257b","host_type":"repository"},{"url":"https://curis.ku.dk/ws/files/322568666/1_s2.0_S0147619X22000336_main.pdf","host_type":"repository"}],"fields_of_study":["Antibiotic Resistance in Bacteria","Bacteriophages and microbial interactions","Bacterial Genetics and Biotechnology","Gene Transfer, Horizontal","Plasmids","Conjugation, Genetic","beta-Lactamases","Anti-Bacterial Agents"],"mesh_terms":["Anti-Bacterial Agents","beta-Lactamases","Conjugation, Genetic","Plasmids","Gene Transfer, Horizontal"],"keywords":["Plasmid","Horizontal gene transfer","Mobile genetic elements","Biology","Genetics","Gene","Transposable element","Transposition (logic)","Bacterial conjugation","Reporter gene","Computational biology","Mutant","Phylogenetics","Gene expression","Genetic engineering","Conjugative Plasmid","Λ Red Recombination System","Tn7 Transposons"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T20:56:44.837934Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}