{"doi":"10.1016/j.peptides.2014.01.011","title":"Characterization of a far-red analog of ghrelin for imaging GHS-R in P19-derived cardiomyocytes","abstract":null,"journal":"Peptides","year":2014,"id":636684,"datarank":0.519860385419959,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"self_citation_contribution":0.519860385419959,"citation_network_contribution":0.0,"self_endowment_contribution":0.519860385419959,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1652666,"name":"Rebecca McGirr","orcid":null,"position":1,"is_corresponding":false},{"id":1652667,"name":"Carlie L. Charlton","orcid":null,"position":2,"is_corresponding":false},{"id":1652668,"name":"Dov B. Kagan","orcid":null,"position":3,"is_corresponding":false},{"id":1652669,"name":"Lisa M. Hoffman","orcid":null,"position":4,"is_corresponding":false},{"id":422358,"name":"Leonard G. Luyt","orcid":"0000-0002-0941-4731","position":5,"is_corresponding":false},{"id":1652670,"name":"Savita Dhanvantari","orcid":null,"position":6,"is_corresponding":false},{"id":1652665,"name":"Gregory A.F. Douglas","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Characterization of a far-red analog of ghrelin for imaging GHS-R in P19-derived cardiomyocytes","abstract":"Ghrelin and its receptor, the growth hormone secretagogue receptor (GHS-R), are expressed in the heart, and may function to promote cardiomyocyte survival, differentiation and contractility. Previously, we had generated a truncated analog of ghrelin conjugated to fluorescein isothiocyanate for the purposes of determining GHS-R expression in situ. We now report the generation and characterization of a far-red ghrelin analog, [Dpr(3)(octanoyl), Lys(19)(Cy5)]ghrelin (1-19), and show that it can be used to image changes in GHS-R in developing cardiomyocytes. We also generated the des-acyl analog, des-acyl [Lys(19)(Cy5)]ghrelin (1-19) and characterized its binding to mouse heart sections. Receptor binding affinity of Cy5-ghrelin as measured in HEK293 cells overexpressing GHS-R1a was within an order of magnitude of that of fluorescein-ghrelin and native human ghrelin, while the des-acyl Cy5-ghrelin did not bind GHS-R1a. Live cell imaging in HEK293/GHS-R1a cells showed cell surface labeling that was displaced by excess ghrelin. Interestingly, Cy5-ghrelin, but not the des-acyl analog, showed concentration-dependent binding in mouse heart tissue sections. We then used Cy5-ghrelin to track GHS-R expression in P19-derived cardiomyocytes. Live cell imaging at different time points after DMSO-induced differentiation showed that GHS-R expression preceded that of the differentiation marker aMHC and tracked with the contractility marker SERCA 2a. Our far-red analog of ghrelin adds to the tools we are developing to map GHS-R in developing and diseased cardiac tissues.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24468548","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"Canadian Institutes of Health Research","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Canadian Institutes of Health Research","grant_id":"","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"Elsevier TDM","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S019697811400014X?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S019697811400014X?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.peptides.2014.01.011","host_type":""},{"url":"https://ir.lib.uwo.ca/cgi/viewcontent.cgi?article=1078&context=biophysicspub","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/24468548","host_type":""},{"url":"https://dx.doi.org/10.1016/j.peptides.2014.01.011","host_type":""},{"url":"https://ir.lib.uwo.ca/context/biophysicspub/article/1078/viewcontent/Characterization_of_a_far_red_analog_of_ghrelin_for_imaging_GHS_R_in.pdf","host_type":""},{"url":"https://doi.org/https://doi.org/10.1016/j.peptides.2014.01.011","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":["Myocardium","Myocytes, Cardiac","Animals","Mice, Inbred C57BL","Humans","Peptide Fragments","Cell Differentiation","Molecular Structure","Amino Acid Sequence","Molecular Sequence Data","Female","Male","Ghrelin","Receptors, Ghrelin","Molecular Imaging","HEK293 Cells"],"keywords":["Fluorescence","Differentiation","Cardiomyocytes","Ghrelin","p19 cells","Ghs-r","Male","Molecular Sequence Data","610","Inbred C57BL","Mice","Receptors","Animals","Humans","Myocytes, Cardiac","Amino Acid Sequence","Receptors, Ghrelin","Myocytes","Molecular Structure","Myocardium","Cell Differentiation","Peptide Fragments","Molecular Imaging","Mice, Inbred C57BL","HEK293 Cells","Medical Biophysics","Female","Cardiac"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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