{"doi":"10.1016/j.pacs.2022.100437","title":"Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging.","abstract":"Near-infrared photoacoustic imaging (NIR-PAI) combines the advantages of optical and ultrasound imaging to provide anatomical and functional information of tissues with high resolution. Although NIR-PAI is promising, its widespread use is hindered by the limited availability of NIR contrast agents. J-aggregates (JA) made of indocyanine green dye (ICG) represents an attractive class of biocompatible contrast agents for PAI. Here, we present a facile synthesis method that combines ICG and ICG-azide dyes for producing contrast agents with tunable size down to 230 nm and direct functionalization with targeting moieties. The ICG-JA platform has a detectable PA signal in vitro that is two times stronger than whole blood and high photostability. The targeting ability of ICG-JA was measured in vitro using HeLa cells. The ICG-JA platform was then injected into mice and in vivo NIR-PAI showed enhanced visualization of liver and spleen for 90 min post-injection with a contrast-to-noise ratio of 2.42.","journal":"Photoacoustics","year":2022,"id":239166,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":51,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9477,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":864469,"name":"Giovanni Giammanco","orcid":"0000-0002-2364-4891","position":1,"is_corresponding":false},{"id":864470,"name":"Chih-Hsiang Hu","orcid":"0000-0002-6068-0728","position":2,"is_corresponding":false},{"id":864471,"name":"Joshua Bush","orcid":"0000-0003-3231-5333","position":3,"is_corresponding":false},{"id":864472,"name":"Leandro Soto Cordova","orcid":"0009-0001-6193-5972","position":4,"is_corresponding":false},{"id":688431,"name":"Dylan J. Lawrence","orcid":"0000-0001-9483-6156","position":5,"is_corresponding":false},{"id":865251,"name":"Jeffrey L. Moran","orcid":null,"position":6,"is_corresponding":false},{"id":864473,"name":"Parag V. Chitnis","orcid":"0000-0003-0845-5619","position":7,"is_corresponding":false},{"id":552749,"name":"Rémi Veneziano","orcid":"0000-0002-2726-3770","position":8,"is_corresponding":false},{"id":864468,"name":"Shrishti Singh","orcid":"0000-0001-5180-3936","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T00:22:36.865157Z","pmid":"36570471","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}