{"doi":"10.1016/j.omtn.2025.102447","title":"Enhancing RNA editing efficiency and specificity with engineered ADAR2 guide RNAs","abstract":null,"journal":"Molecular Therapy Nucleic Acids","year":2025,"id":618326,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":263214,"name":"Sheng Ding","orcid":"0000-0002-3354-1263","position":1,"is_corresponding":false},{"id":1032006,"name":"Shan Zhou","orcid":"0000-0002-6476-3280","position":2,"is_corresponding":false},{"id":390275,"name":"Feng Du","orcid":"0000-0003-0333-2687","position":3,"is_corresponding":false},{"id":703825,"name":"Shuai Liu","orcid":"0000-0003-3129-7073","position":4,"is_corresponding":false},{"id":506419,"name":"Xin Cui","orcid":"0000-0002-4277-7972","position":5,"is_corresponding":false},{"id":1208650,"name":"Juan Dong","orcid":"0000-0002-3070-9972","position":6,"is_corresponding":false},{"id":629785,"name":"Xin Huang","orcid":"0000-0002-6208-4751","position":7,"is_corresponding":false},{"id":1431344,"name":"Zhuo Tang","orcid":"0000-0001-9081-8153","position":8,"is_corresponding":false},{"id":1595043,"name":"Xilei Ai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Enhancing RNA editing efficiency and specificity with engineered ADAR2 guide RNAs","abstract":"RNA editing is a prospective therapeutic approach for correcting harmful mutations, offering the benefits of reversibility and tunability without permanently modifying the genome. However, the relatively low enzymatic activity and the occurrence of off-target editing events present significant challenges, limiting its utility. In response to this limitation, we introduced a novel strategy: strand displacement-responsive ADAR system for RNA editing (SPRING) by adding a \"blocking sequence\" to form a hairpin guide RNA. This modification significantly improves the efficiency of site-directed RNA editing (SDRE) at various target sites. Furthermore, the use of hairpin guide RNA within the SPRING system enhances the specificity of RNA editing through competitive reactions during target hybridization. In principle, this approach can be employed across various ADAR-based editing systems, offering a novel RNA-editing platform with wide-ranging potential for research, therapy, and biotech applications.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39967855","pmcid":"PMC11834095","openalex_id":"https://openalex.org/W4406320189","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"22177111","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"22377124","title":null},{"funder_name":"Sichuan Provincial Science and Technology Support Program","grant_id":"2023NSFSC0016","title":null},{"funder_name":"Sichuan Provincial Science and Technology Support Program","grant_id":"2022YFS0432","title":null},{"funder_name":"Sichuan Provincial Science and Technology Support Program","grant_id":"2022YFS0012","title":null}],"total_grants":5,"fwci":3.6633,"citation_percentile":0.92953857,"influential_citations":0,"citation_trend":[{"year":2025,"count":6},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1016/j.omtn.2025.102447","host_type":"journal"},{"url":"https://doi.org/10.1016/j.omtn.2025.102447","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2162253125000010?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2162253125000010?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39967855","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11834095","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11834095/pdf/main.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11834095","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11834095?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["RNA regulation and disease","CRISPR and Genetic Engineering","RNA and protein synthesis mechanisms"],"mesh_terms":[],"keywords":["RNA","Computational biology","RNA editing","Computer science","Biology","Genetics","Gene","Spring","Specificity","Efficiency","Mt: Rna/Dna Editing","Blocking Sequence"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T04:05:13.835572Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}