{"doi":"10.1016/j.omtm.2020.01.013","title":"Development and Preclinical Evaluation of an Integrase Defective Lentiviral Vector Vaccine Expressing the HIVACAT T Cell Immunogen in Mice","abstract":"Cellular immune responses play a fundamental role in controlling viral replication and AIDS progression in human immunodeficiency virus (HIV)-infected subjects and in simian immunodeficiency virus (SIV)-infected macaques. Integrase defective lentiviral vector (IDLV) represents a promising vaccine candidate, inducing functional and durable immune responses in mice and non-human primates. Here, we designed HIV- and SIV-based IDLVs to express the HIVACAT T cell immunogen (HTI), a mosaic antigen designed to cover vulnerable sites in HIV-1 Gag, Pol, Vif, and Nef. We observed that HTI expression during lentiviral vector production interfered profoundly with IDLV particles release because of sequestration of both HIV- and SIV-Gag proteins in the cytoplasm of the vector-producing cells. However, modifications in IDLV design and vector production procedures greatly improved recovery of both HIV- and SIV-based IDLV-HTI. Immunization experiments in BALB/c mice showed that both IDLVs elicited HTI-specific T cell responses. However, immunization with HIV-based IDLV elicited also a T cell response toward exogenous HIV proteins in IDLV particles, suggesting that SIV-based IDLV may be a preferable platform to assess the induction of transgene-specific immune responses against rationally designed HIV structural antigens. These data support the further evaluation of IDLV as an effective platform of T cell immunogens for the development of an effective HIV vaccine.","journal":"Molecular Therapy — Methods & Clinical Development","year":2020,"id":79223,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9551,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":411714,"name":"Martina Borghi","orcid":"0000-0001-6313-0273","position":1,"is_corresponding":false},{"id":411715,"name":"Maria Franca Pirillo","orcid":"0000-0002-6186-692X","position":2,"is_corresponding":false},{"id":286584,"name":"Serena Cecchetti","orcid":"0000-0002-3601-176X","position":3,"is_corresponding":false},{"id":411716,"name":"Roberta Bona","orcid":"0000-0002-9226-3734","position":4,"is_corresponding":false},{"id":411717,"name":"Andrea Canitano","orcid":"0000-0002-1494-1237","position":5,"is_corresponding":false},{"id":411718,"name":"Zuleika Michelini","orcid":"0000-0001-6841-7396","position":6,"is_corresponding":false},{"id":412783,"name":"Antonio Di Virgilio","orcid":null,"position":7,"is_corresponding":false},{"id":411719,"name":"Àlex Olvera","orcid":"0000-0002-9934-5759","position":8,"is_corresponding":false},{"id":97914,"name":"Christian Brander","orcid":"0000-0002-0548-5778","position":9,"is_corresponding":false},{"id":407460,"name":"Donatella Negri","orcid":"0000-0002-3437-9288","position":10,"is_corresponding":false},{"id":407465,"name":"Andrea Cara","orcid":"0000-0003-4967-1895","position":11,"is_corresponding":false},{"id":411713,"name":"Alessandra Gallinaro","orcid":"0000-0001-7618-8896","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-18T21:50:33.748601Z","pmid":"32154327","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}