{"doi":"10.1016/j.molcel.2023.12.021","title":"Sequencing of N6-methyl-deoxyadenosine at single-base resolution across the mammalian genome","abstract":"-methyl-deoxyadenosine (6mA) is abundant in bacteria and protists, its presence and function in mammalian genomes have been less clear. We present Direct-Read 6mA sequencing (DR-6mA-seq), an antibody-independent method, to measure 6mA at base resolution. DR-6mA-seq employs a unique mutation-based strategy to reveal 6mA sites as misincorporation signatures without any chemical or enzymatic modulation of 6mA. We validated DR-6mA-seq through the successful mapping of the well-characterized G(6mA)TC motif in the E. coli DNA. As expected, when applying DR-6mA-seq to mammalian systems, we found that genomic DNA (gDNA) 6mA abundance is generally low in most mammalian tissues and cells; however, we did observe distinct gDNA 6mA sites in mouse testis and glioblastoma cells. DR-6mA-seq provides an enabling tool to detect 6mA at single-base resolution for a comprehensive understanding of DNA 6mA in eukaryotes.","journal":"Molecular Cell","year":2024,"id":430247,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":239946,"name":"Xiaolong Cui","orcid":"0000-0002-1345-2271","position":1,"is_corresponding":false},{"id":243495,"name":"Li-Sheng Zhang","orcid":null,"position":2,"is_corresponding":false},{"id":841799,"name":"Chang Ye","orcid":"0000-0003-0236-5671","position":3,"is_corresponding":false},{"id":264157,"name":"Pingluan Wang","orcid":"0000-0002-7817-7709","position":4,"is_corresponding":false},{"id":1209611,"name":"Yuhao Zhong","orcid":"0009-0001-5980-0454","position":5,"is_corresponding":false},{"id":239945,"name":"Tong Wu","orcid":"0000-0001-9968-9909","position":6,"is_corresponding":false},{"id":262495,"name":"Zhong Zheng","orcid":"0000-0002-3500-9510","position":7,"is_corresponding":false},{"id":21408,"name":"Chuan He","orcid":"0000-0003-4319-7424","position":8,"is_corresponding":false},{"id":634888,"name":"Xinran Feng","orcid":"0000-0002-6106-8645","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-19T01:59:16.092672Z","pmid":"38215754","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}