{"doi":"10.1016/j.molcel.2022.06.027","title":"Structure of a backtracked hexasomal intermediate of nucleosome transcription","abstract":null,"journal":"Molecular Cell","year":2022,"id":652883,"datarank":0.6394019815561974,"base_score":4.2626798770413155,"endowment":4.2626798770413155,"self_citation_contribution":0.6394019815561974,"citation_network_contribution":0.0,"self_endowment_contribution":0.6394019815561974,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":70,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1703351,"name":"Moritz Ochmann","orcid":null,"position":1,"is_corresponding":false},{"id":1616265,"name":"Gaurika Garg","orcid":null,"position":2,"is_corresponding":false},{"id":58317,"name":"Seychelle M. Vos","orcid":"0000-0003-1985-2994","position":3,"is_corresponding":false},{"id":713287,"name":"Patrick Cramer","orcid":"0000-0001-5454-7755","position":4,"is_corresponding":false},{"id":58318,"name":"Lucas Farnung","orcid":"0000-0002-8200-2493","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Structure of a backtracked hexasomal intermediate of nucleosome transcription","abstract":"During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage when the human elongation factors DSIF, PAF1 complex (PAF), RTF1, SPT6, and TFIIS are present. The cryo-EM structure of an intermediate of the nucleosome passage shows a partially unraveled hexasome that lacks the proximal H2A-H2B dimer and interacts with the RNA Pol II jaw, DSIF, and the CTR9trestle helix. RNA Pol II adopts a backtracked state with the RNA 3' end dislodged from the active site and bound in the RNA Pol II pore. Additional structures and biochemical data show that human TFIIS enters the RNA Pol II pore and stimulates the cleavage of the backtracked RNA and nucleosome passage.","is_dataset_classified":null,"base_score":4.2626798770413155,"endowment":4.2626798770413155,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35858621","pmcid":null,"openalex_id":"https://openalex.org/W4286257123","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"882357","title":"Structural biology of chromatin transcription"}],"total_grants":1,"fwci":4.7026,"citation_percentile":0.96553922,"influential_citations":0,"citation_trend":[{"year":2022,"count":3},{"year":2023,"count":21},{"year":2024,"count":19},{"year":2025,"count":17},{"year":2026,"count":10}],"oa_status":"closed","license":"Elsevier Non-Commercial","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1097276522006104?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1097276522006104?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.molcel.2022.06.027","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35858621","host_type":"repository"},{"url":"https://resolver.sub.uni-goettingen.de/purl?gro-2/113146","host_type":""},{"url":"https://mbexc.uni-goettingen.de/literature/publications/527","host_type":""},{"url":"https://hdl.handle.net/21.11116/0000-000B-19E7-5","host_type":""},{"url":"https://hdl.handle.net/21.11116/0000-000B-19E9-3","host_type":""},{"url":"http://dx.doi.org/10.1016/j.molcel.2022.06.027","host_type":""}],"fields_of_study":["RNA modifications and cancer","RNA and protein synthesis mechanisms","RNA Research and Splicing","0301 basic medicine","0303 health sciences","03 medical and health sciences","Cell Nucleus","Humans","Nucleosomes","RNA","RNA Polymerase II","Transcription Factors","Transcription, Genetic","Transcriptional Elongation Factors"],"mesh_terms":["Cell Nucleus","Humans","Nucleosomes","RNA","RNA Polymerase II","Transcription Factors","Transcription, Genetic","Transcriptional Elongation Factors"],"keywords":["RNA polymerase II","RNA","Biology","Transcription (linguistics)","Nucleosome","RNA polymerase I","RNA polymerase","Cell biology","Molecular biology","RNA polymerase III","Cleavage (geology)","Transcription factor II D","DNA","Chromatin","Biochemistry","Gene","Gene expression","Promoter","Transcription","Nucleus","Transcription Elongation","Backtracking","Rna Pol Ii","Tfiis","H2a-h2b Dimer","Hexasome","Nucleosome Passage","Cell Nucleus","Transcription, Genetic","Humans","Transcriptional Elongation Factors","Nucleosomes","Transcription Factors"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T17:22:04.279291Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}