{"doi":"10.1016/j.molcel.2022.05.005","title":"Structural basis of transposon end recognition explains central features of Tn7 transposition systems","abstract":"Tn7 is a bacterial transposon with relatives containing element-encoded CRISPR-Cas systems mediating RNA-guided transposon insertion. Here, we present the 2.7 Å cryoelectron microscopy structure of prototypic Tn7 transposase TnsB interacting with the transposon end DNA. When TnsB interacts across repeating binding sites, it adopts a beads-on-a-string architecture, where the DNA-binding and catalytic domains are arranged in a tiled and intertwined fashion. The DNA-binding domains form few base-specific contacts leading to a binding preference that requires multiple weakly conserved sites at the appropriate spacing to achieve DNA sequence specificity. TnsB binding imparts differences in the global structure of the protein-bound DNA ends dictated by the spacing or overlap of binding sites explaining functional differences in the left and right ends of the element. We propose a model of the strand-transfer complex in which the terminal TnsB molecule is rearranged so that its catalytic domain is in a position conducive to transposition.","journal":"Molecular Cell","year":2022,"id":247463,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":885043,"name":"Mariusz Czarnocki‐Cieciura","orcid":"0000-0001-5582-2851","position":1,"is_corresponding":false},{"id":885471,"name":"Karolina M. Górecka-Minakowska","orcid":null,"position":2,"is_corresponding":false},{"id":885044,"name":"Robert J. Wingo","orcid":"0000-0002-0831-3234","position":3,"is_corresponding":false},{"id":885472,"name":"Justyna Jackiewicz","orcid":null,"position":4,"is_corresponding":false},{"id":885045,"name":"Weronika Zajko","orcid":"0000-0001-9915-0305","position":5,"is_corresponding":false},{"id":383921,"name":"Jarosław Poznański","orcid":"0000-0003-2684-1775","position":6,"is_corresponding":false},{"id":885046,"name":"Michał Rawski","orcid":"0000-0002-8553-7637","position":7,"is_corresponding":false},{"id":815241,"name":"Timothy Grant","orcid":"0000-0002-4855-8703","position":8,"is_corresponding":false},{"id":279045,"name":"Joseph E. Peters","orcid":"0000-0001-8309-3297","position":9,"is_corresponding":false},{"id":415758,"name":"Marcin Nowotny","orcid":"0000-0001-8632-0977","position":10,"is_corresponding":false},{"id":885042,"name":"Zuzanna Kaczmarska","orcid":"0000-0002-7668-1442","position":0,"is_corresponding":true}],"reference_count":65,"raw_metadata":null,"created_at":"2026-07-19T00:23:53.369530Z","pmid":"35654042","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}