{"doi":"10.1016/j.molcel.2021.11.004","title":"Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery","abstract":null,"journal":"Molecular Cell","year":2022,"id":628922,"datarank":0.6681520944380261,"base_score":4.454347296253507,"endowment":4.454347296253507,"self_citation_contribution":0.6681520944380261,"citation_network_contribution":0.0,"self_endowment_contribution":0.6681520944380261,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":85,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1416961,"name":"Süleyman Bozkurt","orcid":"0000-0003-4965-3195","position":1,"is_corresponding":false},{"id":884504,"name":"Jonas B. Michaelis","orcid":"0000-0001-9548-2337","position":2,"is_corresponding":false},{"id":1628615,"name":"Kevin Klann","orcid":"0000-0003-2276-8128","position":3,"is_corresponding":false},{"id":881076,"name":"Christian Münch","orcid":"0000-0003-3832-090X","position":4,"is_corresponding":false},{"id":1628614,"name":"Jasmin Adriana Schäfer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery","abstract":"Most mitochondrial proteins are translated in the cytosol and imported into mitochondria. Mutations in the mitochondrial protein import machinery cause human pathologies. However, a lack of suitable tools to measure protein uptake across the mitochondrial proteome has prevented the identification of specific proteins affected by import perturbation. Here, we introduce mePROD<sup>mt</sup>, a pulsed-SILAC based proteomics approach that includes a booster signal to increase the sensitivity for mitochondrial proteins selectively, enabling global dynamic analysis of endogenous mitochondrial protein uptake in cells. We applied mePROD<sup>mt</sup> to determine protein uptake kinetics and examined how inhibitors of mitochondrial import machineries affect protein uptake. Monitoring changes in translation and uptake upon mitochondrial membrane depolarization revealed that protein uptake was extensively modulated by the import and translation machineries via activation of the integrated stress response. Strikingly, uptake changes were not uniform, with subsets of proteins being unaffected or decreased due to changes in translation or import capacity.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34847359","pmcid":"PMC8791276","openalex_id":null,"authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"259130777","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"403765277","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"390339347","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"MU 4216/1-1","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB 1177","title":null},{"funder_name":"European Research Council","grant_id":"803565","title":null},{"funder_name":"European Research Council","grant_id":"","title":null},{"funder_name":"Seventh Framework Programme","grant_id":"","title":null}],"total_grants":8,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":null,"license":"http://creativecommons.org/licenses/by/4.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1097276521009540?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1097276521009540?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.molcel.2021.11.004","host_type":"Unpaywall"},{"url":"https://europepmc.org/articles/PMC8791276","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Hela Cells","Mitochondria","Humans","Carbonyl Cyanide m-Chlorophenyl Hydrazone","Electron Transport Complex I","Mitochondrial Membrane Transport Proteins","Mitochondrial Proteins","Proteome","Uncoupling Agents","Proteomics","Protein Biosynthesis","Protein Transport","Kinetics","Female"],"keywords":["Mitochondria","Translation","Protein translocation","Disease","Proteomics","integrated stress response","Respiratory Chain Complexes","Proteostasis","SILAC","Tmt"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"pxd"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T15:36:04.841287Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}