{"doi":"10.1016/j.molcel.2020.06.015","title":"The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates","abstract":"Meiotic recombination proceeds via binding of RPA, RAD51, and DMC1 to single-stranded DNA (ssDNA) substrates created after formation of programmed DNA double-strand breaks. Here we report high-resolution in vivo maps of RPA and RAD51 in meiosis, mapping their binding locations and lifespans to individual homologous chromosomes using a genetically engineered hybrid mouse. Together with high-resolution microscopy and DMC1 binding maps, we show that DMC1 and RAD51 have distinct spatial localization on ssDNA: DMC1 binds near the break site, and RAD51 binds away from it. We characterize inter-homolog recombination intermediates bound by RPA in vivo, with properties expected for the critical displacement loop (D-loop) intermediates. These data support the hypothesis that DMC1, not RAD51, performs strand exchange in mammalian meiosis. RPA-bound D-loops can be resolved as crossovers or non-crossovers, but crossover-destined D-loops may have longer lifespans. D-loops resemble crossover gene conversions in size, but their extent is similar in both repair pathways.","journal":"Molecular Cell","year":2020,"id":51148,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":166,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9383,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":254572,"name":"Philipp Becker","orcid":"0000-0001-6901-6527","position":1,"is_corresponding":false},{"id":254573,"name":"Tao Li","orcid":"0000-0002-5728-3721","position":2,"is_corresponding":false},{"id":254574,"name":"Daniela Moralli","orcid":"0000-0001-7977-8496","position":3,"is_corresponding":false},{"id":254575,"name":"Gang Zhang","orcid":"0000-0003-2951-8287","position":4,"is_corresponding":false},{"id":254576,"name":"Clare Bycroft","orcid":"0000-0002-1139-0732","position":5,"is_corresponding":false},{"id":254577,"name":"Catherine Green","orcid":"0000-0002-7068-7106","position":6,"is_corresponding":false},{"id":254578,"name":"Scott Keeney","orcid":"0000-0002-1283-6417","position":7,"is_corresponding":false},{"id":254579,"name":"Qinghua Shi","orcid":"0000-0003-1180-9799","position":8,"is_corresponding":false},{"id":254580,"name":"Benjamin Davies","orcid":"0000-0003-3623-600X","position":9,"is_corresponding":false},{"id":5158,"name":"Peter Donnelly","orcid":"0000-0001-9495-3408","position":10,"is_corresponding":false},{"id":62181,"name":"Anjali Gupta Hinch","orcid":"0000-0002-0836-3418","position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":null,"created_at":"2026-07-18T20:40:28.278215Z","pmid":"32610038","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}