{"doi":"10.1016/j.molcel.2018.10.018","title":"Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor","abstract":null,"journal":"Molecular Cell","year":2018,"id":589660,"datarank":1.6043570698498608,"base_score":3.9889840465642745,"endowment":3.9889840465642745,"self_citation_contribution":0.5983476069846413,"citation_network_contribution":1.0060094628652196,"self_endowment_contribution":0.5983476069846413,"citer_contribution":1.0060094628652196,"corpus_percentile":null,"corpus_rank":null,"citation_count":53,"citer_count":42,"citers_with_citation_signal":37,"citers_with_endowment":37,"datacite_reuse_total":6,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1508641,"name":"Harshil Patel","orcid":null,"position":1,"is_corresponding":false},{"id":141484,"name":"Minghao Chia","orcid":null,"position":2,"is_corresponding":false},{"id":141482,"name":"Fabien Moretto","orcid":null,"position":3,"is_corresponding":false},{"id":145292,"name":"David Frith","orcid":null,"position":4,"is_corresponding":false},{"id":145294,"name":"Ambrosius P. Snijders","orcid":null,"position":5,"is_corresponding":false},{"id":684973,"name":"Folkert J. van Werven","orcid":"0000-0002-6685-2084","position":6,"is_corresponding":false},{"id":1508640,"name":"Andrew C.K. Wu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor","abstract":"Many active eukaryotic gene promoters exhibit divergent noncoding transcription, but the mechanisms restricting expression of these transcripts are not well understood. Here, we demonstrate how a sequence-specific transcription factor represses divergent noncoding transcription at highly expressed genes in yeast. We find that depletion of the transcription factor Rap1 induces noncoding transcription in a large fraction of Rap1-regulated gene promoters. Specifically, Rap1 prevents transcription initiation at cryptic promoters near its binding sites, which is uncoupled from transcription regulation in the protein-coding direction. We further provide evidence that Rap1 acts independently of previously described chromatin-based mechanisms to repress cryptic or divergent transcription. Finally, we show that divergent transcription in the absence of Rap1 is elicited by the RSC chromatin remodeler. We propose that a sequence-specific transcription factor limits access of basal transcription machinery to regulatory elements and adjacent sequences that act as divergent cryptic promoters, thereby providing directionality toward productive transcription.","is_dataset_classified":null,"base_score":3.9889840465642745,"endowment":3.9889840465642745,"datacite_reuse_total":6,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30576656","pmcid":"PMC6310685","openalex_id":"https://openalex.org/W2949808355","authors":[],"funders":[{"funder_name":"Francis Crick Institute","grant_id":"FC001203","title":null},{"funder_name":"The Francis Crick Institute","grant_id":"10011","title":null},{"funder_name":"Cancer Research UK","grant_id":"17576","title":null},{"funder_name":"The Francis Crick Institute","grant_id":"10002","title":null},{"funder_name":"The Francis Crick Institute","grant_id":"10203","title":null},{"funder_name":"Wellcome 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