{"doi":"10.1016/j.molcel.2009.05.018","title":"EDEM1 Recognition and Delivery of Misfolded Proteins to the SEL1L-Containing ERAD Complex","abstract":null,"journal":"Molecular Cell","year":2009,"id":651870,"datarank":0.7401710899696038,"base_score":4.9344739331306915,"endowment":4.9344739331306915,"self_citation_contribution":0.7401710899696038,"citation_network_contribution":0.0,"self_endowment_contribution":0.7401710899696038,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":138,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":188602,"name":"Taku Tamura","orcid":"0000-0001-6074-9273","position":1,"is_corresponding":false},{"id":1700260,"name":"Johan C. Sunryd","orcid":null,"position":2,"is_corresponding":false},{"id":320512,"name":"Daniel N. Hebert","orcid":"0000-0003-1537-4446","position":3,"is_corresponding":false},{"id":1700259,"name":"James H. Cormier","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"EDEM1 Recognition and Delivery of Misfolded Proteins to the SEL1L-Containing ERAD Complex","abstract":"Terminally misfolded or unassembled secretory proteins are retained in the endoplasmic reticulum (ER) and subsequently cleared by the ER-associated degradation (ERAD) pathway. The degradation of ERAD substrates involves mannose trimming of N-linked glycans; however, the mechanisms of substrate recognition and sorting to the ERAD pathway are poorly defined. EDEM1 (ER degradation-enhancing alpha-mannosidase-like 1 protein) has been proposed to play a role in ERAD substrate signaling or recognition. We show that EDEM1 specifically binds nonnative proteins in a glycan-independent manner. Inhibition of mannosidase activity with kifunensine or disruption of the EDEM1 mannosidase-like domain by mutation had no effect on EDEM1 substrate binding but diminished its association with the ER membrane adaptor protein SEL1L. These results support a model whereby EDEM1 binds nonnative proteins and uses its mannosidase-like domain to target aberrant proteins to the ER membrane dislocation and ubiquitination complex containing SEL1L.","is_dataset_classified":null,"base_score":4.9344739331306915,"endowment":4.9344739331306915,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19524542","pmcid":"PMC2740909","openalex_id":"https://openalex.org/W2153693056","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA79864","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008515","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA079864","title":null}],"total_grants":3,"fwci":8.6422,"citation_percentile":0.98409869,"influential_citations":0,"citation_trend":[{"year":2012,"count":13},{"year":2013,"count":11},{"year":2014,"count":13},{"year":2015,"count":5},{"year":2016,"count":7},{"year":2017,"count":2},{"year":2018,"count":5},{"year":2019,"count":8},{"year":2020,"count":11},{"year":2021,"count":5},{"year":2022,"count":4},{"year":2023,"count":5},{"year":2024,"count":5},{"year":2025,"count":1},{"year":2026,"count":4}],"oa_status":"bronze","license":"https://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"http://www.cell.com/article/S1097276509003517/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S1097276509003517/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1097276509003517?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1097276509003517?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.molcel.2009.05.018","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19524542","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2740909","host_type":"repository"},{"url":"https://works.bepress.com/daniel_hebert/8","host_type":"repository"}],"fields_of_study":["Lysosomal Storage Disorders Research","Endoplasmic Reticulum Stress and Disease","Cellular transport and secretion"],"mesh_terms":["Binding Sites","Cell Line","Endoplasmic Reticulum","Glycoproteins","Humans","Mannose","Membrane Proteins","Polysaccharides","Proteins","Substrate Specificity","Protein Structure, Tertiary","Protein Folding","Carbohydrate Metabolism"],"keywords":["Endoplasmic-reticulum-associated protein degradation","Endoplasmic reticulum","Biology","Cell biology","Mannosidase","Protein folding","Glycan","Signal transducing adaptor protein","Membrane protein","Protein targeting","Secretory pathway","Mannose","Unfolded protein response","Biochemistry","Glycoprotein","Signal transduction","Golgi apparatus","Membrane"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T11:04:50.061462Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}