{"doi":"10.1016/j.molcel.2004.05.019","title":"Phosphorylation of RhoGDI by Pak1 Mediates Dissociation of Rac GTPase","abstract":null,"journal":"Molecular Cell","year":2004,"id":676832,"datarank":0.8208406010507213,"base_score":5.472270673671475,"endowment":5.472270673671475,"self_citation_contribution":0.8208406010507213,"citation_network_contribution":0.0,"self_endowment_contribution":0.8208406010507213,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":237,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1768417,"name":"Andreas Schnelzer","orcid":null,"position":1,"is_corresponding":false},{"id":201826,"name":"Gary M. Bokoch","orcid":null,"position":2,"is_corresponding":false},{"id":1768415,"name":"Céline DerMardirossian","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Phosphorylation of RhoGDI by Pak1 Mediates Dissociation of Rac GTPase","abstract":"Selective activation of Rac GTPase signaling pathways requires the specific release of Rac from RhoGDI complexes. We identified a RhoGDI kinase from bovine brain as p21-activated kinase (Pak). Pak1 binds and phosphorylates RhoGDI both in vitro and in vivo at Ser101 and Ser174. This resulted in dissociation of Rac1-RhoGDI, but not RhoA-RhoGDI, complexes, as determined by in vitro assays of complexation and in vivo by coimmunoprecipitation analysis. We observed that Cdc42-induced Rac1 activation is inhibited by expression of Pak1 autoinhibitory domain. The dissociation of Rac1 from RhoGDI and its subsequent activation stimulated by PDGF or EGF is also attenuated by Pak1 autoinhibitory domain, and this is dependent on the ability of RhoGDI to be phosphorylated at Ser101/174. These results support a role for Pak1-mediated RhoGDI phosphorylation as a mechanism for Cdc42-mediated Rac activation, and suggest the possibility of Rac-induced positive feed-forward regulation of Rac activity.","is_dataset_classified":null,"base_score":5.472270673671475,"endowment":5.472270673671475,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15225553","pmcid":null,"openalex_id":"https://openalex.org/W2024309614","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"HL48008","title":null},{"funder_name":"National Institutes of Health","grant_id":"2R01HL048008-14","title":"G Protein Regulation of the Neutrophil NADPH Oxidase"}],"total_grants":2,"fwci":6.6984,"citation_percentile":0.97830047,"influential_citations":0,"citation_trend":[{"year":2012,"count":11},{"year":2013,"count":10},{"year":2014,"count":8},{"year":2015,"count":10},{"year":2016,"count":7},{"year":2017,"count":6},{"year":2018,"count":8},{"year":2019,"count":6},{"year":2020,"count":7},{"year":2021,"count":14},{"year":2022,"count":2},{"year":2023,"count":11},{"year":2024,"count":8},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"bronze","license":"Elsevier Non-Commercial","oa_locations":[{"url":"http://www.cell.com/article/S1097276504003053/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S1097276504003053/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1097276504003053?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1097276504003053?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.molcel.2004.05.019","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15225553","host_type":"repository"},{"url":"http://dx.doi.org/10.1016/j.molcel.2004.05.019","host_type":""},{"url":"https://dx.doi.org/10.1016/j.molcel.2004.05.019","host_type":""}],"fields_of_study":["Protein Kinase Regulation and GTPase Signaling","Metabolism, Diabetes, and Cancer","Cell death mechanisms and regulation","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Binding Sites","Cattle","Epidermal Growth Factor","Guanine Nucleotide Dissociation Inhibitors","HeLa Cells","Humans","Phosphorylation","Platelet-Derived Growth Factor","Protein Serine-Threonine Kinases","Protein Structure, Tertiary","Signal Transduction","cdc42 GTP-Binding Protein","p21-Activated Kinases","rac GTP-Binding Proteins","rac1 GTP-Binding Protein","rho Guanine Nucleotide Dissociation Inhibitor alpha","rho-Specific Guanine Nucleotide Dissociation Inhibitors"],"mesh_terms":["Animals","Binding Sites","Cattle","Epidermal Growth Factor","HeLa Cells","Humans","Phosphorylation","Platelet-Derived Growth Factor","Signal Transduction","Protein Serine-Threonine Kinases","Protein Structure, Tertiary","Guanine Nucleotide Dissociation Inhibitors","rac GTP-Binding Proteins","cdc42 GTP-Binding Protein","rac1 GTP-Binding Protein","p21-Activated Kinases","rho-Specific Guanine Nucleotide Dissociation Inhibitors","rho Guanine Nucleotide Dissociation Inhibitor alpha","Hela Cells"],"keywords":["PAK1","RAC1","CDC42","RHOA","Cell biology","Biology","Phosphorylation","GTPase","Rac GTP-Binding Proteins","Small GTPase","Signal transduction","Kinase","Immunoprecipitation","Cell culture","Genetics","Platelet-Derived Growth Factor","rac1 GTP-Binding Protein","rho Guanine Nucleotide Dissociation Inhibitor alpha","Binding Sites","Epidermal Growth Factor","Protein Serine-Threonine Kinases","Protein Structure, Tertiary","p21-Activated Kinases","Animals","Humans","rho-Specific Guanine Nucleotide Dissociation Inhibitors","Cattle","cdc42 GTP-Binding Protein","Molecular Biology","Guanine Nucleotide Dissociation Inhibitors","HeLa Cells"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T03:10:14.023987Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}