{"doi":"10.1016/j.mocell.2024.100158","title":"An RXLR effector disrupts vesicle trafficking at ER-Golgi interface for Phytophthora capsici pathogenicity","abstract":"Phytophthora species, an oomycete plant pathogen, secrete effectors into plant cells throughout their life cycle for manipulating host immunity to achieve successful colonization. However, the molecular mechanisms underlying effector-triggered necrotic cell death remain elusive. In this study, we identified an RXLR (amino acid residue; Arginine-Any amino acid-Leucine-Arginine motif) effector (Pc12) from Phytophthora capsici, which contributes to virulence and induces necrosis by triggering a distinct endoplasmic reticulum (ER) stress response through its interaction with Rab13-2. The necrotic cell death induced by Pc12 did not exhibit conventional effector-triggered immunity-mediated hypersensitive cell death, including the involvement of nucleotide-binding site leucine-rich repeat downstream signaling components and transcriptional reprogramming of defense-related genes. Instead, it alters the localization of ER-resident proteins and confines secretory proteins within the ER. Pc12 directly interacts with Rab13-2, which is primarily localized to the ER and Golgi apparatus, resulting in a diminished Rab13-2 signal on the Golgi apparatus. Furthermore, Rab13-2 exhibits increased affinity for its interactor, Rab escort protein 1, in the presence of Pc12. Structural predictions revealed that a specific residue of Rab13-2 is crucial for binding to the C-terminus of Pc12. Substitution of this residue reduced its interaction with Pc12 and impaired P. capsici infection while maintaining its interaction with Rab escort protein 1 and prenylated Rab acceptor 1. These findings provide insight into how a pathogen effector induces a distinct form of necrotic cell death to facilitate colonization of the host plant by disrupting the recycling of Rab13-2, a protein involved in vesicle trafficking at the ER-Golgi interface.","journal":"Molecules and Cells","year":2024,"id":458829,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9602,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1179332,"name":"Jesse Kaleku","orcid":"0000-0002-7089-1609","position":1,"is_corresponding":false},{"id":1179337,"name":"Haeun Kim","orcid":"0000-0002-6171-0650","position":2,"is_corresponding":false},{"id":1179336,"name":"Minji Kang","orcid":"0000-0001-8118-3184","position":3,"is_corresponding":false},{"id":1179335,"name":"Hui Jeong Kang","orcid":"0000-0002-1347-8965","position":4,"is_corresponding":false},{"id":1179333,"name":"Jongchan Woo","orcid":"0000-0002-2994-6444","position":5,"is_corresponding":false},{"id":1179334,"name":"Hongshi Jin","orcid":"0000-0003-3283-1012","position":6,"is_corresponding":false},{"id":1179338,"name":"Seungmee Jung","orcid":"0000-0003-2660-7663","position":7,"is_corresponding":false},{"id":74953,"name":"Cécile Segonzac","orcid":"0000-0002-5537-7556","position":8,"is_corresponding":false},{"id":1179339,"name":"Eunsook Park","orcid":"0000-0003-2984-3039","position":9,"is_corresponding":false},{"id":1179340,"name":"Doil Choi","orcid":"0000-0002-4366-3627","position":10,"is_corresponding":false},{"id":886710,"name":"Jihyun Kim","orcid":"0000-0003-0195-4319","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-19T02:03:50.608887Z","pmid":"39577746","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}