{"doi":"10.1016/j.micpath.2021.105353","title":"Infection with a hypervirulent strain of Helicobacter pylori primes gastric cells toward intestinal transdifferentiation","abstract":null,"journal":"Microbial Pathogenesis","year":2022,"id":614758,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":900695,"name":"Maryam Esmaeili","orcid":"0000-0002-6203-4332","position":1,"is_corresponding":false},{"id":1584239,"name":"Mohammad Tashakoripour","orcid":null,"position":2,"is_corresponding":false},{"id":1584240,"name":"Mahmoud Eshagh Hosseini","orcid":null,"position":3,"is_corresponding":false},{"id":188979,"name":"Hossein Baharvand","orcid":"0000-0001-6528-3687","position":4,"is_corresponding":false},{"id":1584241,"name":"Marjan Mohammadi","orcid":null,"position":5,"is_corresponding":false},{"id":1265604,"name":"Samaneh Saberi","orcid":"0000-0002-5246-9092","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Infection with a hypervirulent strain of Helicobacter pylori primes gastric cells toward intestinal transdifferentiation","abstract":"<h4>Background</h4>Intestinal metaplasia, gastric-to-intestinal transdifferentiation, occurs as a result of the misexpression of certain regulatory factors, leading to genetic reprogramming. Here, we have evaluated the H. pylori-induced expression patterns of these candidate genes.<h4>Methods</h4>The expression levels of 1) tissue-specific transcription factors (RUNX3, KLF5, SOX2, SALL4, CDX1 and CDX2), 2) stemness factors (TNFRSF19, LGR5, VIL1) and 3) tissue-specific mucins (MUC5AC, MUC2) were evaluated by quantitative real-time PCR in gastric primary cells (GPCs), in parallel with two gastric cancer (MKN45 and AGS) cell lines, up to 96h following H. pylori infection.<h4>Results</h4>Following H. pylori infection of GPCs, RUNX3 declined at 24h post infection (-6.2 ± 0.3) and remained downregulated for up to 96h. Subsequently, overexpression of self-renewal and pluripotency transcription factors, KLF5 (3.6 ± 0.2), SOX2 (7.6 ± 0.5) and SALL4 (4.3 ± 0.2) occurred. The expression of TNFRSF19 and LGR5, demonstrated opposing trends, with an early rise of the former (4.5 ± 0.3) at 8h, and a simultaneous fall of the latter (-1.8 ± 0.5). This trend was reversed at 96h, with the decline in TNFRSF19 (-5.5 ± 0.2), and escalation of LGR5 (2.6 ± 0.2) and VIL1 (1.8 ± 0.3). Ultimately, CDX1 and CDX2 were upregulated by 1.9 and 4.7-fold, respectively. The above scenario was, variably observed in MKN45 and AGS cells.<h4>Conclusion</h4>Our data suggests an interdependent gene regulatory network, induced by H. pylori infection. This interaction begins with the downregulation of RUNX3, upregulation of self-renewal and pluripotency transcription factors, KLF5, SOX2 and SALL4, leading to the downregulation of TNFRSF19, upregulation of LGR5 and aberrant expression of intestine-specific transcription factors, potentially facilitating the process of gastric-to-intestinal transdifferentiation.","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34896202","pmcid":null,"openalex_id":"https://openalex.org/W4225620581","authors":[],"funders":[{"funder_name":"Royan Institute","grant_id":"94000090","title":null},{"funder_name":"Pasteur Institute of Iran","grant_id":"-9121","title":null},{"funder_name":"Pasteur Institute of Iran","grant_id":"722","title":null},{"funder_name":"Pasteur Institute of Iran","grant_id":"","title":null}],"total_grants":4,"fwci":0.4051,"citation_percentile":0.64006586,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"closed","license":"http://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0882401021006276?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0882401021006276?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.micpath.2021.105353","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34896202","host_type":"repository"}],"fields_of_study":["Helicobacter pylori-related gastroenterology studies","Cancer Cells and Metastasis","Wnt/β-catenin signaling in development and cancer","CDX2 Transcription Factor","Cell Transdifferentiation","Gastric Mucosa","Helicobacter Infections","Helicobacter pylori","Humans","Intestines","Receptors, Tumor Necrosis Factor","Stomach Neoplasms"],"mesh_terms":["CDX2 Transcription Factor","Gastric Mucosa","Humans","Intestines","Stomach Neoplasms","Helicobacter pylori","Helicobacter Infections","Receptors, Tumor Necrosis Factor","Cell Transdifferentiation"],"keywords":["LGR5","CDX2","Downregulation and upregulation","Biology","Transdifferentiation","SOX2","Reprogramming","Helicobacter pylori","Intestinal metaplasia","KLF4","Transcription factor","Epigenetics","Cancer research","Mucin 2","Gastric mucosa","Stem cell","Cell biology","Gene expression","Gene","Genetics","Stomach","Cancer stem cell","Gastric cancer","Wnt Signalling"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T16:28:13.499702Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}