{"doi":"10.1016/j.mex.2020.101161","title":"Flash-comet assay","abstract":null,"journal":"MethodsX","year":2020,"id":665546,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1737952,"name":"Björn Hellman","orcid":"0000-0002-3429-7856","position":1,"is_corresponding":false},{"id":1737951,"name":"Erik Bivehed","orcid":"0000-0003-2471-1432","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Flash-comet assay","abstract":"In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the unwinding and electrophoresis. This allows a higher voltage during the electrophoresis (5 V/cm instead of 0.7 V/cm), making it possible to reduce the unwinding time from 20 to 40 to 2.5 min, and the electrophoresis time from 10 to 20 to 1 min. Still, the Flash-comet was found to detect DNA strand breaks and alkali-labile sites with a higher degree of sensitivity than the conventional protocol in cells that had been exposed to H 2 O 2 or ionizing radiation. In order to prevent alkaline hydrolysis of DNA, the wash and lysis solutions have been modified in the Flash-comet protocol. •By using an alkaline LiOH-based medium, the Flash-comet allows for much shorter times for both unwinding and electrophoresis than the conventional comet assay without compromising the sensitivity. •The reduced run-times of the unwinding and electrophoresis steps in the Flash-comet should also reduce the risk of laboratory-induced alkaline hydrolysis of DNA when evaluating the potential DNA-damaging effects of different types of xenobiotics.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33304837","pmcid":"PMC7718155","openalex_id":"https://openalex.org/W3106731454","authors":[],"funders":[{"funder_name":"Uppsala Universitet","grant_id":"","title":null}],"total_grants":1,"fwci":0.2829,"citation_percentile":0.46138091,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"http://methods-x.com/article/S2215016120303812/pdf","host_type":"journal"},{"url":"http://methods-x.com/article/S2215016120303812/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2215016120303812?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2215016120303812?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.mex.2020.101161","host_type":"journal"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7718155","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33304837","host_type":"repository"},{"url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-434712","host_type":"repository"},{"url":"https://doaj.org/article/aec968ad76ac410e8802798fda3d81e6","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7718155","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7718155?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Carcinogens and Genotoxicity Assessment","Effects and risks of endocrine disrupting chemicals","Genetically Modified Organisms Research"],"mesh_terms":[],"keywords":["Comet assay","Comet","Electrophoresis","Gel electrophoresis","Flash (photography)","DNA damage","Chemistry","Lysis","DNA","Alkaline lysis","Chromatography","Biochemistry","Biology","Physics","DNA-damage","Single Cell Analysis","Genotoxicity Testing","In Vitro Comet Assay","Low Conductivity Electrophoresis Solution","Gentle Lysis"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T11:26:39.827923Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}