{"doi":"10.1016/j.mcpro.2024.100760","title":"The One Hour Human Proteome","abstract":"We describe deep analysis of the human proteome in less than one hour. We achieve this expedited proteome characterization by leveraging state-of-the-art sample preparation, chromatographic separations, data analysis tools, and by using the new Orbitrap Astral mass spectrometer equipped with a quadrupole mass filter, a high-field Orbitrap mass analyzer, and an asymmetric track lossless (Astral) mass analyzer. The system offers high MS/MS acquisition speed of 200 Hz and detects hundreds of peptide sequences per second within data independent- or data-dependent acquisition modes of operation. The fast-switching capabilities of the new quadrupole complement the sensitivity and fast ion scanning of the Astral analyzer to enable narrow-bin data-independent analysis (DIA) methods. Over a 30-minute active chromatographic method consuming a total analysis time of 56 minutes, the Q-Orbitrap-Astral hybrid MS collects an average of 4,319 MS1 scans and 438,062 MS/MS scans per run, producing 235,916 peptide sequences (1% false discovery rate (FDR)). On average, each 30-minute analysis achieved detection of 10,411 protein groups (1% FDR). We conclude, with these results and alongside other recent reports, that the one-hour human proteome is within reach.","journal":"Molecular & Cellular Proteomics","year":2024,"id":418187,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":54,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.6254,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":109556,"name":"Trenton M. Peters-Clarke","orcid":"0000-0002-9153-2525","position":1,"is_corresponding":false},{"id":1014930,"name":"Tabiwang N. Arrey","orcid":"0009-0001-2888-2055","position":2,"is_corresponding":false},{"id":1014929,"name":"Eugen Damoc","orcid":"0000-0002-4422-2866","position":3,"is_corresponding":false},{"id":1161639,"name":"Margaret Lea Robinson","orcid":"0000-0002-4019-3770","position":4,"is_corresponding":false},{"id":1161636,"name":"Noah M. Lancaster","orcid":"0009-0002-3609-7951","position":5,"is_corresponding":false},{"id":109552,"name":"Evgenia Shishkova","orcid":"0000-0001-6097-6097","position":6,"is_corresponding":false},{"id":1206449,"name":"C. Moss","orcid":null,"position":7,"is_corresponding":false},{"id":1015698,"name":"Anna Pashkova","orcid":null,"position":8,"is_corresponding":false},{"id":85038,"name":"Pavel Sinitcyn","orcid":"0000-0002-2653-1702","position":9,"is_corresponding":false},{"id":109566,"name":"Dain R. Brademan","orcid":"0000-0002-0971-9711","position":10,"is_corresponding":false},{"id":1205637,"name":"Scott T. Quarmby","orcid":"0000-0003-2991-5637","position":11,"is_corresponding":false},{"id":1015701,"name":"Amelia C. Peterson","orcid":null,"position":12,"is_corresponding":false},{"id":633542,"name":"Martin Zeller","orcid":null,"position":13,"is_corresponding":false},{"id":1015703,"name":"Daniel Hermanson","orcid":null,"position":14,"is_corresponding":false},{"id":1014933,"name":"Hamish Stewart","orcid":"0009-0000-1510-4353","position":15,"is_corresponding":false},{"id":1015702,"name":"Christian Hock","orcid":null,"position":16,"is_corresponding":false},{"id":247291,"name":"Alexander Makarov","orcid":"0000-0002-7046-6709","position":17,"is_corresponding":false},{"id":247290,"name":"Vlad Zabrouskov","orcid":"0000-0003-3567-9407","position":18,"is_corresponding":false},{"id":71208,"name":"Joshua J. Coon","orcid":"0000-0002-0004-8253","position":19,"is_corresponding":false},{"id":109568,"name":"Lia R. Serrano","orcid":"0000-0001-5662-5884","position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":null,"created_at":"2026-07-19T01:57:02.483590Z","pmid":"38579929","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}