{"doi":"10.1016/j.mcpro.2023.100705","title":"Phosphorylation Dynamics in a flg22-Induced, G Protein–Dependent Network Reveals the AtRGS1 Phosphatase","abstract":"The microbe-associated molecular pattern flg22 is recognized in a flagellin-sensitive 2-dependent manner in root tip cells. Here, we show a rapid and massive change in protein abundance and phosphorylation state of the Arabidopsis root cell proteome in WT and a mutant deficient in heterotrimeric G-protein-coupled signaling. flg22-induced changes fall on proteins comprising a subset of this proteome, the heterotrimeric G protein interactome, and on highly-populated hubs of the immunity network. Approximately 95% of the phosphorylation changes in the heterotrimeric G-protein interactome depend, at least partially, on a functional G protein complex. One member of this interactome is ATBα, a substrate-recognition subunit of a protein phosphatase 2A complex and an interactor to Arabidopsis thaliana Regulator of G Signaling 1 protein (AtRGS1), a flg22-phosphorylated, 7-transmembrane spanning modulator of the nucleotide-binding state of the core G-protein complex. A null mutation of ATBα strongly increases basal endocytosis of AtRGS1. AtRGS1 steady-state protein level is lower in the atbα mutant in a proteasome-dependent manner. We propose that phosphorylation-dependent endocytosis of AtRGS1 is part of the mechanism to degrade AtRGS1, thus sustaining activation of the heterotrimeric G protein complex required for the regulation of system dynamics in innate immunity. The PP2A(ATBα) complex is a critical regulator of this signaling pathway.","journal":"Molecular & Cellular Proteomics","year":2023,"id":359972,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9475,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":173936,"name":"Christian Montes","orcid":"0000-0003-1249-2308","position":1,"is_corresponding":false},{"id":315483,"name":"Natalie M. Clark","orcid":"0000-0003-0988-321X","position":2,"is_corresponding":false},{"id":173935,"name":"Gaoyuan Song","orcid":"0000-0003-1633-9159","position":3,"is_corresponding":false},{"id":818922,"name":"Celio Cabral Oliveira","orcid":"0000-0002-0885-5308","position":4,"is_corresponding":false},{"id":315212,"name":"Bharat Mishra","orcid":"0000-0002-7505-5722","position":5,"is_corresponding":false},{"id":819389,"name":"Libuse Brachova","orcid":null,"position":6,"is_corresponding":false},{"id":819390,"name":"Clara M. Seifert","orcid":null,"position":7,"is_corresponding":false},{"id":819391,"name":"Malek S. Mitchell","orcid":null,"position":8,"is_corresponding":false},{"id":1111004,"name":"Jing Yang","orcid":"0000-0001-8940-5845","position":9,"is_corresponding":false},{"id":818923,"name":"Pedro A. B. Reis","orcid":"0000-0003-2396-3662","position":10,"is_corresponding":false},{"id":607997,"name":"Daisuke Urano","orcid":"0000-0003-4541-1076","position":11,"is_corresponding":false},{"id":819392,"name":"M. Shahid Muktar","orcid":null,"position":12,"is_corresponding":false},{"id":173938,"name":"Justin W Walley","orcid":"0000-0001-7553-2237","position":13,"is_corresponding":false},{"id":544161,"name":"Alan M. Jones","orcid":"0000-0002-2365-6462","position":14,"is_corresponding":false},{"id":818921,"name":"Justin M. Watkins","orcid":"0000-0003-4127-1950","position":0,"is_corresponding":true}],"reference_count":116,"raw_metadata":null,"created_at":"2026-07-19T01:13:57.361401Z","pmid":"38135118","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}