{"doi":"10.1016/j.mce.2024.112405","title":"An optimized fractionation method reveals insulin-induced membrane surface localization of GLUT1 to increase glycolysis in LβT2 cells","abstract":"Insulin is an important regulator of whole-body glucose homeostasis. In insulin sensitive tissues such as muscle and adipose, insulin induces the translocation of glucose transporter 4 (GLUT4) to the cell membrane, thereby increasing glucose uptake. However, insulin also signals in tissues that are not generally associated with glucose homeostasis. In the human reproductive endocrine axis, hyperinsulinemia suppresses the secretion of gonadotropins from gonadotrope cells of the anterior pituitary, thereby linking insulin dysregulation to suboptimal reproductive health. In the mouse, gonadotropes express the insulin receptor which has the canonical signaling response of IRS, AKT, and mTOR activation. However, the functional outcomes of insulin action on gonadotropes are unclear. Here, we demonstrate through use of an optimized cell fractionation protocol that insulin stimulation of the LβT2 gonadotropic cell line results in the unexpected translocation of GLUT1 to the plasma membrane. Using our high purity fractionation protocol, we further demonstrate that though Akt signaling in response to insulin is intact, insulin-induced translocation of GLUT1 occurs independently of Akt activation in LβT2 cells. • Optimized fractionation protocol allows quantification of subcellular localization. • Insulin increases GLUT1 trafficking to the membrane in LΒT2 cells. • Insulin increases glycolysis in LΒT2. • Insulin-induced GLUT1 trafficking is Akt independent.","journal":"Molecular and Cellular Endocrinology","year":2024,"id":505534,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1356740,"name":"Kiara Dominique Wiggins","orcid":null,"position":1,"is_corresponding":false},{"id":1356741,"name":"Anjali Varma","orcid":null,"position":2,"is_corresponding":false},{"id":1356742,"name":"Zena Del Mundo","orcid":null,"position":3,"is_corresponding":false},{"id":1356743,"name":"José Antonio Adame","orcid":null,"position":4,"is_corresponding":false},{"id":607686,"name":"Alyssa J. Cozzo","orcid":"0000-0001-6690-4748","position":5,"is_corresponding":false},{"id":1356444,"name":"Oscar Muñoz","orcid":"0000-0002-5262-7476","position":6,"is_corresponding":false},{"id":1356744,"name":"Uyen-Vy Le","orcid":null,"position":7,"is_corresponding":false},{"id":1356745,"name":"Davina Trinh","orcid":null,"position":8,"is_corresponding":false},{"id":1356746,"name":"Alexis C Garcia","orcid":null,"position":9,"is_corresponding":false},{"id":471098,"name":"Metztli Cisneros‐Aguirre","orcid":"0000-0002-3260-113X","position":10,"is_corresponding":false},{"id":910577,"name":"Monica L. Gonzalez Ramirez","orcid":null,"position":11,"is_corresponding":false},{"id":1001720,"name":"Jeremiah Keyes","orcid":"0000-0003-4253-6834","position":12,"is_corresponding":false},{"id":232973,"name":"Jin Zhang","orcid":"0000-0001-7145-7823","position":13,"is_corresponding":false},{"id":426816,"name":"Mark A. Lawson","orcid":"0000-0003-2303-3086","position":14,"is_corresponding":false},{"id":247561,"name":"JoAnn Trejo","orcid":"0000-0003-4405-6228","position":15,"is_corresponding":false},{"id":88595,"name":"Dequina Nicholas","orcid":"0000-0003-4996-2190","position":16,"is_corresponding":false},{"id":328116,"name":"Olivia Molinar‐Inglis","orcid":"0000-0002-4974-9608","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-19T02:10:47.170978Z","pmid":"39481749","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}