{"doi":"10.1016/j.mce.2015.01.015","title":"Notch inhibits chondrogenic differentiation of mesenchymal progenitor cells by targeting Twist1","abstract":null,"journal":"Molecular and Cellular Endocrinology","year":2015,"id":683797,"datarank":0.6261580904843456,"base_score":4.174387269895637,"endowment":4.174387269895637,"self_citation_contribution":0.6261580904843456,"citation_network_contribution":0.0,"self_endowment_contribution":0.6261580904843456,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":64,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":16,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":638330,"name":"Ying Xu","orcid":"0000-0001-7341-8676","position":1,"is_corresponding":false},{"id":943334,"name":"Qin Fu","orcid":"0000-0001-9056-8449","position":2,"is_corresponding":false},{"id":1786346,"name":"Martin Chang","orcid":null,"position":3,"is_corresponding":false},{"id":539166,"name":"Yongjun Wang","orcid":"0000-0002-2801-4573","position":4,"is_corresponding":false},{"id":435429,"name":"Xifu Shang","orcid":"0000-0002-6001-4434","position":5,"is_corresponding":false},{"id":42731,"name":"Chao Wan","orcid":"0000-0001-8643-6662","position":6,"is_corresponding":false},{"id":1786347,"name":"John V. Marymont","orcid":null,"position":7,"is_corresponding":false},{"id":435430,"name":"Yufeng Dong","orcid":"0000-0001-9966-455X","position":8,"is_corresponding":false},{"id":845883,"name":"Ye Tian","orcid":"0000-0002-6846-5141","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Notch inhibits chondrogenic differentiation of mesenchymal progenitor cells by targeting Twist1","abstract":"While Notch signaling plays a critical role in the regulation of cartilage formation, its downstream targets are unknown. To address this we performed gain and losses of function experiments and demonstrate that Notch inhibition of chondrogenesis acts via up-regulation of the transcription factor Twist1. Upon Notch activation, murine limb bud mesenchymal progenitor cells in micromass culture displayed an inhibition of chondrogenesis. Twist1 was found to be exclusively expressed in mesenchymal progenitor cells at the onset stage of chondrogenesis during Notch activation. Inhibition of Notch signaling in these cells significantly reduced protein expression of Twist1. Furthermore, the inhibition effect of NICD1 on MPC chondrogenesis was markedly reduced by knocking down of Twist1. Constitutively active Notch signaling significantly enhanced Twist1 promoter activity; whereas mutation studies indicated that a putative NICD/RBPjK binding element in the promoter region is required for the Notch-responsiveness of the Twist1 promoter. Finally, chromatin immunoprecipitation assays further confirmed that the Notch intracellular domain influences Twist1 by directly binding to the Twist1 promoter. These data provide a novel insight into understanding the molecular mechanisms behind Notch inhibition of the onset of chondrogenesis.","is_dataset_classified":null,"base_score":4.174387269895637,"endowment":4.174387269895637,"datacite_reuse_total":16,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25596548","pmcid":null,"openalex_id":"https://openalex.org/W2095548276","authors":[],"funders":[{"funder_name":"Natural Science Foundation of Liaoning Province","grant_id":"2013021051","title":null},{"funder_name":"Program for Changjiang Scholars and Innovative Research Team in University","grant_id":"IRT1270","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"81220108027","title":null},{"funder_name":"National Institutes of Health","grant_id":"R21AR063803","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2010CB530400","title":null}],"total_grants":5,"fwci":3.6425,"citation_percentile":0.92838615,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2016,"count":5},{"year":2017,"count":3},{"year":2018,"count":6},{"year":2019,"count":6},{"year":2020,"count":4},{"year":2021,"count":5},{"year":2022,"count":1},{"year":2023,"count":7},{"year":2024,"count":22},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"green","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4337804","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4337804","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S0303720715000192?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0303720715000192?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.mce.2015.01.015","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25596548","host_type":"repository"}],"fields_of_study":["Osteoarthritis Treatment and Mechanisms","Developmental Biology and Gene Regulation","Cancer-related molecular mechanisms research","Animals","Binding Sites","Cell Differentiation","Chondrocytes","Chondrogenesis","Embryo, Mammalian","Gene Expression Regulation, Developmental","Immunoglobulin J Recombination Signal Sequence-Binding Protein","Limb Buds","Mesenchymal Stem Cells","Mice","Nuclear Proteins","Primary Cell Culture","Protein Binding","Protein Structure, Tertiary","RNA, Small Interfering","Receptor, Notch1","Signal Transduction","Twist-Related Protein 1"],"mesh_terms":["Animals","Binding Sites","Cell Differentiation","Embryo, Mammalian","Nuclear Proteins","Protein Binding","Signal Transduction","Protein Structure, Tertiary","Gene Expression Regulation, Developmental","Limb Buds","Chondrocytes","Chondrogenesis","RNA, Small Interfering","Mice","Immunoglobulin J Recombination Signal Sequence-Binding Protein","Twist-Related Protein 1","Receptor, Notch1","Mesenchymal Stem Cells","Primary Cell Culture"],"keywords":["Chondrogenesis","Notch signaling pathway","Cell biology","Mesenchymal stem cell","Transcription factor","Biology","Progenitor cell","Signal transduction","Chemistry","Stem cell","Genetics","Gene","Mesenchymal progenitor cell","Notch signaling","Twist1"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.26689583.v1","title":"Additional file 8 of Zebrafish as a model to investigate a biallelic gain-of-function variant in MSGN1, associated with a novel skeletal dysplasia syndrome","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26689583","title":"Additional file 8 of Zebrafish as a model to investigate a biallelic gain-of-function variant in MSGN1, associated with a novel skeletal dysplasia syndrome","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26689580","title":"Additional file 7 of Zebrafish as a model to investigate a biallelic gain-of-function variant in MSGN1, associated 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