{"doi":"10.1016/j.lfs.2022.120848","title":"RSL1D1 modulates cell senescence and proliferation via regulation of PPARγ mRNA stability","abstract":null,"journal":"Life Sciences","year":2022,"id":623291,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1610760,"name":"Fengxin Hao","orcid":null,"position":1,"is_corresponding":false},{"id":233411,"name":"Feng Zhu","orcid":"0000-0002-8131-1219","position":2,"is_corresponding":false},{"id":450970,"name":"Fuwen Yuan","orcid":"0000-0001-5764-2504","position":3,"is_corresponding":false},{"id":1398165,"name":"Liwei Ma","orcid":"0000-0002-7867-8060","position":4,"is_corresponding":false},{"id":609995,"name":"Guodong Li","orcid":"0000-0003-3255-4852","position":5,"is_corresponding":false},{"id":252214,"name":"Jun Chen","orcid":"0000-0002-1273-5624","position":6,"is_corresponding":false},{"id":1610761,"name":"Tanjun Tong","orcid":null,"position":7,"is_corresponding":false},{"id":1179323,"name":"Zhe Jiang","orcid":"0000-0002-3576-6976","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"RSL1D1 modulates cell senescence and proliferation via regulation of PPARγ mRNA stability","abstract":"<h4>Aims</h4>In this study, we will examine if RSL1D1 influences PPARγ expression and explore the underlying mechanism that RSL1D1 regulates PPARγ expression. Moreover, the significance of RSL1D1-PPARγ pathway in cell senescence and proliferation will also be determined.<h4>Main methods</h4>Our main methods include western blotting, immunoprecipitation (IP), real-time PCR, RNA Immunoprecipitation (RIP), biotin-labeled RNA pull down assay, dual luciferase reporter gene assay, senescence-associated β-galactosidase staining, cell proliferation assay, colony formation assay, wound healing assay, blood biochemistry test and Oil red O staining.<h4>Key findings</h4>By analyzing gene chip results we find that the expression of RSL1D1 and PPARγ might be correlated. Then we show that RSL1D1 is a posttranscriptional regulator of PPARγ. RSL1D1 overexpression elevates, while RSL1D1 knockdown inhibits, PPARγ mRNA and protein expression levels. Mechanistically, we find that RSL1D1 directly interacts with the 3'-untranslated region of PPARγ mRNA, and then promotes its stability and increases PPARγ protein expression level. We further demonstrate that RSL1D1 modulates cellular senescence and cell proliferation partially via PPARγ-regulated downstream target genes such as PTEN/p27, NF-κB, GLUT4, and ACL. Moreover, we find that RSL1D1 regulates PPARγ expression and function in a HuR-dependent manner. Last, we show that RSL1D1 knockout in mouse adipose tissue shortens mouse lifespan and leads to hepatic damage which may impair liver damage repair function.<h4>Significance</h4>Collectively, our findings unveil a novel posttranscriptional regulation of PPARγ by RSL1D1 and uncover a critical role of RSL1D1-PPARγ-PPARγ downstream target genes in regulating cellular senescence and cell proliferation.","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35940221","pmcid":null,"openalex_id":"https://openalex.org/W4291015587","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"81772949","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2013CB530801","title":null}],"total_grants":2,"fwci":0.7054,"citation_percentile":0.65795198,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":4},{"year":2026,"count":5}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0024320522005483?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0024320522005483?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.lfs.2022.120848","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35940221","host_type":"repository"}],"fields_of_study":["Peroxisome Proliferator-Activated Receptors","Cytokine Signaling Pathways and Interactions","Protein Kinase Regulation and GTPase Signaling","Animals","Mice","3' Untranslated Regions","beta-Galactosidase","Biotin","Cell Proliferation","Cellular Senescence","NF-kappa B","PPAR gamma","Pregnancy Proteins","Ribosomal Proteins","RNA Stability","RNA, Messenger"],"mesh_terms":["Animals","beta-Galactosidase","Biotin","Pregnancy Proteins","Ribosomal Proteins","RNA, Messenger","NF-kappa B","Cellular Senescence","3' Untranslated Regions","RNA Stability","PPAR gamma","Cell Proliferation","Mice"],"keywords":["Gene knockdown","Cell biology","Cell growth","Biology","Senescence","Immunoprecipitation","Gene expression","Peroxisome proliferator-activated receptor","Regulation of gene expression","Reporter gene","Three prime untranslated region","Messenger RNA","Molecular biology","Untranslated region","Cell culture","Gene","Biochemistry","Genetics","Cell senescence","PPARγ","Post transcriptional regulation","CSIG","Hur"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T23:10:24.841528Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}