{"doi":"10.1016/j.jviromet.2012.03.007","title":"Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus","abstract":null,"journal":"Journal of Virological Methods","year":2012,"id":603999,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1549548,"name":"Shan Peng","orcid":null,"position":1,"is_corresponding":false},{"id":253209,"name":"Pixi Yan","orcid":null,"position":2,"is_corresponding":false},{"id":1549552,"name":"Jiewen Zhou","orcid":null,"position":3,"is_corresponding":false},{"id":1549555,"name":"Qiaoyang Teng","orcid":null,"position":4,"is_corresponding":false},{"id":89756,"name":"Guoxin Li","orcid":"0000-0003-2773-7048","position":5,"is_corresponding":false},{"id":24071,"name":"Xuesong Li","orcid":"0000-0002-1157-0266","position":6,"is_corresponding":false},{"id":1549558,"name":"Zejun Li","orcid":"0009-0000-5052-2171","position":7,"is_corresponding":false},{"id":673959,"name":"Liping Yan","orcid":"0000-0001-5559-7842","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus","abstract":"Duck Tembusu virus (DTMUV) has caused huge losses to the poultry industry in China since the spring of 2010. The development of a rapid, convenient, and reliable method to diagnose this emerging duck infectious disease is critical. In the present study, a real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was compared with the real-time reverse transcription polymerase chain reaction (RT-PCR) for detection of DTMUV. The sensitivity of real-time RT-LAMP was equal to that of the real-time RT-PCR, with a detection limit of 0.01 ELD(50) (50% egg lethal dose). The specificity of the real-time RT-LAMP and real-time RT-PCR was confirmed using RNAs and DNAs extracted from related viruses which cause duck infections. The reproducibility of the real-time RT-PCR assay was better than that of the real-time RT-LAMP. Only three results from 96 tissue samples differed between the real-time RT-LAMP and this real-time RT-PCR, confirming the reliability of these methods. This study indicated that the real-time RT-LAMP is simpler, less time-consuming, and more convenient than the real-time RT-PCR. With its high sensitivity, specificity, and convenience, the real-time RT-LAMP is a practical molecular diagnostic method for rapid and quantitative detection of DTMUV infection in a resource-limited setting.","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22445388","pmcid":null,"openalex_id":"https://openalex.org/W2059288109","authors":[],"funders":[],"total_grants":0,"fwci":1.4065,"citation_percentile":0.79881883,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":4},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0166093412000754?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0166093412000754?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jviromet.2012.03.007","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22445388","host_type":"repository"}],"fields_of_study":["Biosensors and Analytical Detection","Animal Virus Infections Studies","Herpesvirus Infections and Treatments","Animals","Bird Diseases","China","Clinical Laboratory Techniques","Ducks","Flavivirus","Flavivirus Infections","Molecular Diagnostic Techniques","Nucleic Acid Amplification Techniques","Real-Time Polymerase Chain Reaction","Reproducibility of Results","Reverse Transcriptase Polymerase Chain Reaction","Sensitivity and Specificity","Veterinary Medicine"],"mesh_terms":["Animals","Bird Diseases","China","Ducks","Flavivirus","Sensitivity and Specificity","Veterinary Medicine","Reproducibility of Results","Flavivirus Infections","Clinical Laboratory Techniques","Reverse Transcriptase Polymerase Chain Reaction","Nucleic Acid Amplification Techniques","Molecular Diagnostic Techniques","Real-Time Polymerase Chain Reaction"],"keywords":["Reverse Transcription Loop-mediated Isothermal Amplification","Real-time polymerase chain reaction","Biology","Reverse transcription polymerase chain reaction","Loop-mediated isothermal amplification","Reverse transcriptase","Virology","Molecular biology","SYBR Green I","Polymerase chain reaction","Detection limit","Chemistry","Messenger RNA","Gene","DNA","Genetics","Chromatography"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Industry, innovation and infrastructure"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T23:11:01.486897Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}