{"doi":"10.1016/j.jsb.2012.01.009","title":"Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei","abstract":null,"journal":"Journal of Structural Biology","year":2012,"id":594412,"datarank":0.611630616585858,"base_score":4.07753744390572,"endowment":4.07753744390572,"self_citation_contribution":0.611630616585858,"citation_network_contribution":0.0,"self_endowment_contribution":0.611630616585858,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":58,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1521610,"name":"Cédric Bouchet-Marquis","orcid":null,"position":1,"is_corresponding":false},{"id":127599,"name":"J. Richard McIntosh","orcid":null,"position":2,"is_corresponding":false},{"id":828704,"name":"Andreas Hoenger","orcid":"0000-0001-9176-6658","position":3,"is_corresponding":false},{"id":1521613,"name":"Keith Gull","orcid":null,"position":4,"is_corresponding":false},{"id":887117,"name":"Johanna L. Höög","orcid":"0000-0003-2162-3816","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei","abstract":"Trypanosoma brucei is a uni-cellular protist that causes African sleeping sickness. These parasites have a flagellum that is attached to the cell body and is indispensible for its motility. The flagellum consists of a canonical 9+2 axoneme and a paraflagellar rod (PFR), an intricate tripartite, fibrous structure that is connected to the axoneme. In this paper we describe results from cryo-electron tomography of unperturbed flagella. This method revealed novel structures that are likely involved in attaching the flagellum to the cell. We also show the first cryo-electron tomographic images of a basal body in situ, revealing electron dense structures inside its triplet microtubules. Sub-tomogram averaging of the PFR revealed that its distal region is organized as an orthorhombic crystal.","is_dataset_classified":null,"base_score":4.07753744390572,"endowment":4.07753744390572,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22285651","pmcid":"PMC3355306","openalex_id":"https://openalex.org/W2100489837","authors":[],"funders":[{"funder_name":"NIH-NCRR","grant_id":"P41-RR000592","title":null},{"funder_name":"NIH-NIGMS","grant_id":"5R01GM80993","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM080993","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"P41 RR000592","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM080993-03","title":"A Clonable High-Density for 3-D Electron Microscopy of Cellular Structures"},{"funder_name":"Wellcome Trust","grant_id":"unidentified","title":"unidentified"},{"funder_name":"National Institutes of Health","grant_id":"5P41RR000592-34","title":"STUDIES OF THE NUCLEUS/VACUOLE JUNCTION IN S. 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