{"doi":"10.1016/j.jpba.2019.03.039","title":"Monitoring of kynurenine pathway metabolites, neurotransmitters and their metabolites in blood plasma and brain tissue of individuals with latent toxoplasmosis","abstract":null,"journal":"Journal of Pharmaceutical and Biomedical Analysis","year":2019,"id":619227,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1597919,"name":"Miloš Mikoška","orcid":null,"position":1,"is_corresponding":false},{"id":1597920,"name":"Kamila Syslová","orcid":"0000-0001-7791-2874","position":2,"is_corresponding":false},{"id":1597921,"name":"Adéla Böhmová","orcid":null,"position":3,"is_corresponding":false},{"id":1597922,"name":"Hana Tejkalová","orcid":null,"position":4,"is_corresponding":false},{"id":1597923,"name":"Lukáš Vacek","orcid":null,"position":5,"is_corresponding":false},{"id":1597924,"name":"Petr Kodym","orcid":null,"position":6,"is_corresponding":false},{"id":1597925,"name":"Daniel Krsek","orcid":null,"position":7,"is_corresponding":false},{"id":1597926,"name":"Jiří Horáček","orcid":null,"position":8,"is_corresponding":false},{"id":1597918,"name":"Jana Vondroušová","orcid":"0000-0003-0569-055X","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Monitoring of kynurenine pathway metabolites, neurotransmitters and their metabolites in blood plasma and brain tissue of individuals with latent toxoplasmosis","abstract":"The aim of the presented work was to develop a highly sensitive, accurate and rapid analytical method for the determination of concentration levels of tryptophan and its metabolites of kynurenine catabolic pathway, as well as neurotransmitters and their metabolites in complex biological matrices (brain tissue and blood plasma). The developed analytical method consists of analytes separation from the biological matrices by protein precipitation (blood plasma) or solvent extraction (brain tissue), derivatization of the analytes and their detection by high-performance liquid chromatography combined with mass spectrometry. Individual steps of the whole process were optimized and the method was validated in the terms of selectivity, linearity (R<sup>2</sup>≥0.980), precision (RSD ≤ 13.3%), recovery (≥82.0%), limit of detection (1.8 ng/mL of blood plasma, 2.2 pg/mg of brain tissue) and limit of quantification (2.5 ng/mL of blood plasma, 2.8 pg/mg of brain tissue). The method was subsequently verified by an animal study, where the concentration levels of the analytes in biological matrices (blood plasma and brain tissue) of T. gondii - infected rats and control animals were compared. All the data obtained from the animal study were statistically evaluated. Increased concentration levels of kynurenine catabolic pathway metabolites (e.g. kynurenine, 3-hydroxykynurenine, quinolinic acid) were observed in the case of T. gondii - infected rats in contrast to the control group. The opposite effect was determined in the case of serotonin and its metabolite 5-hydroxyindoleacetic acid, where higher concentration levels were found in blood plasma of healthy subjects. Finally, Principal Component Analysis (PCA) was utilized for a score plot formation. PCA score plots have demonstrated the similarities of individuals within each group and the differences among the groups.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30925271","pmcid":null,"openalex_id":"https://openalex.org/W2921491219","authors":[],"funders":[{"funder_name":"specific university research","grant_id":"21-SVV/2018","title":null},{"funder_name":"OPPC","grant_id":"CZ.2.16/3.1.00/21537","title":null},{"funder_name":"NPU I","grant_id":"LO1601","title":null},{"funder_name":"NPU I","grant_id":"LO1611","title":null},{"funder_name":"AZV MHCR","grant_id":"17-31852A","title":null}],"total_grants":5,"fwci":1.2858,"citation_percentile":0.74678497,"influential_citations":0,"citation_trend":[{"year":2019,"count":2},{"year":2020,"count":2},{"year":2021,"count":4},{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0731708518325718?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0731708518325718?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jpba.2019.03.039","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30925271","host_type":"repository"}],"fields_of_study":["Toxoplasma gondii Research Studies","Tryptophan and brain disorders","Pediatric health and respiratory diseases","Animals","Brain","Chromatography, High Pressure Liquid","Hydroxyindoleacetic Acid","Kynurenine","Male","Neurotransmitter Agents","Plasma","Quinolinic Acid","Rats","Rats, Wistar","Serotonin","Tandem Mass Spectrometry","Toxoplasmosis","Tryptophan"],"mesh_terms":["Animals","Brain","Chromatography, High Pressure Liquid","Hydroxyindoleacetic Acid","Kynurenine","Male","Plasma","Serotonin","Toxoplasmosis","Tryptophan","Rats, Wistar","Quinolinic Acid","Neurotransmitter Agents","Rats","Tandem Mass Spectrometry"],"keywords":["Chemistry","Kynurenine","Toxoplasmosis","Metabolite","Kynurenine pathway","Pharmacology","Toxoplasma gondii","Blood plasma","Biochemistry","Immunology","Tryptophan","Antibody","Amino acid","Principal component analysis","Hplc-esi-ms/ms","Latent Toxoplasmosis","Kynurenine Catabolic Pathway"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T06:26:12.105287Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}