{"doi":"10.1016/j.joen.2021.11.005","title":"Single-Cell RNA Sequencing Analysis of Human Dental Pulp Stem Cell and Human Periodontal Ligament Stem Cell","abstract":null,"journal":"Journal of Endodontics","year":2022,"id":596957,"datarank":0.6141516843333151,"base_score":4.0943445622221,"endowment":4.0943445622221,"self_citation_contribution":0.6141516843333151,"citation_network_contribution":0.0,"self_endowment_contribution":0.6141516843333151,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":59,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1529048,"name":"Dongzi Chen","orcid":null,"position":1,"is_corresponding":false},{"id":1529049,"name":"Munsu Park","orcid":null,"position":2,"is_corresponding":false},{"id":1529050,"name":"Sunil Kim","orcid":null,"position":3,"is_corresponding":false},{"id":1529051,"name":"Yoon Jeong Choi","orcid":null,"position":4,"is_corresponding":false},{"id":1384996,"name":"Seok Jun Moon","orcid":"0000-0001-7282-2888","position":5,"is_corresponding":false},{"id":79900,"name":"Dong Min Shin","orcid":"0000-0001-6042-0435","position":6,"is_corresponding":false},{"id":107585,"name":"Ji Hyun Lee","orcid":"0000-0003-3810-9186","position":7,"is_corresponding":false},{"id":1529052,"name":"Euiseong Kim","orcid":"0000-0003-2126-4761","position":8,"is_corresponding":false},{"id":1529047,"name":"Sukjoon Lee","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Single-Cell RNA Sequencing Analysis of Human Dental Pulp Stem Cell and Human Periodontal Ligament Stem Cell","abstract":"<h4>Introduction</h4>Many studies have investigated the transcriptome profiles of dental stem cells for regenerative medicine. However, such studies use bulk RNA and do not consider cell-level heterogeneity. Here, we investigated the characteristics and heterogeneity of human dental pulp stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) at the single-cell level and examined the differences between them.<h4>Methods</h4>hDPSCs and hPDLSCs were obtained from caries-free premolars (n = 2). Single-cell RNA sequencing of hDPSCs and hPDLSCs was performed using a Single Emulsion Systems (Dolomite Microfluidics, Royston, UK) droplet microfluidic device and the Illumina NextSeq550 system (Illumina, San Diego, CA). Data alignment was performed using STAR v2.4.0, and further analysis was performed using the Seurat, singleR, and clusterProfiler packages in R (R Foundation for Statistical Computing, Vienna, Austria).<h4>Results</h4>hDPSCs and hPDLSCs were separated into 3 clusters. hDPSCs mainly exhibited osteogenic and neurogenic cell populations. The main populations of hPDLSCs comprised osteogenic and myofibroblastic populations. hPDLSCs showed high \"scores\" for osteogenic gene expression, whereas hDPSCs had high neurogenic and endogenic scores.<h4>Conclusions</h4>Single-cell RNA sequencing of hDPSCs and hPDLSCs revealed that the genes were expressed in specific clusters. The results of these analyses can be used as reference databases and valuable resources for further research in dental therapeutics.","is_dataset_classified":null,"base_score":4.0943445622221,"endowment":4.0943445622221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34801591","pmcid":null,"openalex_id":"https://openalex.org/W3217150840","authors":[],"funders":[{"funder_name":"National Research Foundation of Korea","grant_id":"2016R1A5A2008630","title":null},{"funder_name":"National Research Foundation of Korea","grant_id":"2021R1A2C2011049","title":null},{"funder_name":"Ministry of Science, ICT and Future Planning","grant_id":"","title":null},{"funder_name":"National Research Foundation of Korea","grant_id":"","title":null}],"total_grants":4,"fwci":2.2957,"citation_percentile":0.89735608,"influential_citations":0,"citation_trend":[{"year":2022,"count":3},{"year":2023,"count":11},{"year":2024,"count":17},{"year":2025,"count":21},{"year":2026,"count":7}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S0099239921007974","host_type":"repository"},{"url":"https://www.sciencedirect.com/science/article/pii/S0099239921007974","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S0099239921007974?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0099239921007974?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.joen.2021.11.005","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34801591","host_type":"repository"}],"fields_of_study":["Single-cell and spatial transcriptomics","Mesenchymal stem cell research","Cancer Cells and Metastasis"],"mesh_terms":["Cell Differentiation","Cells, Cultured","Dental Pulp","Humans","Osteogenesis","Periodontal Ligament","Stem Cells","Sequence Analysis, RNA","Cell Proliferation"],"keywords":["Periodontal ligament stem cells","Dental pulp stem cells","Stem cell","Transcriptome","Cell","Biology","Cell biology","Gene","Gene expression","Genetics","Alkaline phosphatase","Biochemistry","Gene expression analysis","Human Periodontal Ligament Stem Cell","Human Dental Pulp Stem Cell","Single-cell Rna Sequencing"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T11:49:37.717383Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}