{"doi":"10.1016/j.joca.2020.10.008","title":"NF-κB-mediated effects on behavior and cartilage pathology in a non-invasive loading model of post-traumatic osteoarthritis","abstract":"OBJECTIVE: This study aimed to examine the temporal activation of NF-κB and its relationship to the development of pain-related sensitivity and behavioral changes in a non-invasive murine knee loading model of PTOA. METHOD: Following knee injury NF-κB activity was assessed longitudinally via in vivo imaging in FVB. Cg-Tg (HIV-EGFP,luc)8Tsb/J mice. Measures of pain-related sensitivity and behavior were also assessed longitudinally for 16 weeks. Additionally, we antagonized NF-κB signaling via intra-articular delivery of an IκB kinase two antagonist to understand how local NF-κB inhibition might alter disease progression. RESULTS: /sr) fold increase in signaling when compared to control joints. Furthermore, injury resulted in the long-term development of hindpaw allodynia. Hyperalgesia withdrawal thresholds were reduced at injured knee joints, with the largest reduction occurring 2 days following injury (estimate of between group difference 129.1 g with 95% CI 60.9,197.4 g), static weight bearing on injured limbs was also reduced. Local delivery of an NF-κB inhibitor following joint injury reduced chondrocyte death and influenced the development of pain-related sensitivity but did not reduce long-term cartilage degeneration. CONCLUSION: These findings underscore the development of behavioral changes in this non-invasive loading model of PTOA and their relationships to NF-κB activation and pathology. They also highlight the potential chondroprotective effects of NF-κB inhibition shortly following joint injury despite limitations in preventing the long-term development of joint degeneration in this model of PTOA.","journal":"Osteoarthritis and Cartilage","year":2020,"id":68781,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9636,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":364357,"name":"Era Jain","orcid":"0000-0002-7172-401X","position":1,"is_corresponding":false},{"id":364358,"name":"Burçin Yavuz","orcid":"0000-0003-2352-9321","position":2,"is_corresponding":false},{"id":364359,"name":"Thomas McGrath","orcid":"0000-0003-4208-6888","position":3,"is_corresponding":false},{"id":118839,"name":"L. CHEN","orcid":null,"position":4,"is_corresponding":false},{"id":285048,"name":"Matthew J. Silva","orcid":"0000-0001-7375-4522","position":5,"is_corresponding":false},{"id":230166,"name":"Gabriel Mbalaviele","orcid":"0000-0003-4660-0952","position":6,"is_corresponding":false},{"id":232645,"name":"Farshid Guilak","orcid":"0000-0001-7380-0330","position":7,"is_corresponding":false},{"id":225373,"name":"David L. Kaplan","orcid":"0000-0002-9245-7774","position":8,"is_corresponding":false},{"id":364360,"name":"Lori A. Setton","orcid":"0000-0001-5992-4206","position":9,"is_corresponding":false},{"id":364356,"name":"Ian M. Berke","orcid":"0000-0002-6639-5506","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T21:41:52.279368Z","pmid":"33246158","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}