{"doi":"10.1016/j.jnutbio.2012.03.019","title":"Glutamate and CO2 production from glutamine in incubated enterocytes of adult and very old rats","abstract":null,"journal":"The Journal of Nutritional Biochemistry","year":2013,"id":591940,"datarank":0.5435688585595849,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.19818109461047798,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.19818109461047798,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":5,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1514602,"name":"Guy Bielicki","orcid":null,"position":1,"is_corresponding":false},{"id":191363,"name":"Anne-Marie Beaufrère","orcid":null,"position":2,"is_corresponding":false},{"id":1514603,"name":"Michelle Mignon","orcid":null,"position":3,"is_corresponding":false},{"id":191365,"name":"Philippe Patureau Mirand","orcid":null,"position":4,"is_corresponding":false},{"id":1514604,"name":"Jean-Pierre Renou","orcid":null,"position":5,"is_corresponding":false},{"id":191366,"name":"Dominique Meynial-Denis","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Glutamate and CO2 production from glutamine in incubated enterocytes of adult and very old rats","abstract":"Glutamine is the major fuel for enterocytes and promotes the growth of intestinal mucosa. Although oral glutamine exerts a positive effect on intestinal villus height in very old rats, how glutamine is used by enterocytes is unclear. Adult (8 months) and very old (27 months) female rats were exposed to intermittent glutamine supplementation for 50% of their age lifetime. Treated rats received glutamine added to their drinking water, and control rats received water alone. Jejunal epithelial cells (~300×10(6) cells) were incubated in oxygenated Krebs-Henseleit buffer for 30 min containing [1-(13)C] glutamine (~17 M) for analysis of glutamine metabolites by (13)C nuclear magnetic resonance ((13)C NMR). An aliquot fraction was incubated in the presence of [U-(14)C] glutamine to measure produced CO2. Glutamine pretreatment increased glutamate production and decreased CO2 production in very old rats. The ratio CO2/glutamate, which was very high in control very old rats, was similar at both ages after glutamine pretreatment, as if enterocytes from very old rats recovered the metabolic abilities of enterocytes from adult rats. Our results suggest that long-term treatment with glutamine started before advanced age (a) prevented the loss of rat body weight without limiting sarcopenia and (b) had a beneficial effect on enterocytes from very old rats probably by favoring the role of glutamate as a precursor for glutathione, arginine and proline biosynthesis, which was not detected in (13)C NMR spectra in our experimental conditions.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22898569","pmcid":null,"openalex_id":"https://openalex.org/W2119738915","authors":[],"funders":[],"total_grants":0,"fwci":0.95,"citation_percentile":0.74495716,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":5},{"year":2016,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0955286312001039?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0955286312001039?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jnutbio.2012.03.019","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22898569","host_type":"repository"},{"url":"https://hal.inrae.fr/hal-02651144","host_type":"repository"}],"fields_of_study":["Diet and metabolism studies","Clinical Nutrition and Gastroenterology","Metabolism and Genetic Disorders","Aging","Animals","Carbon Dioxide","Enterocytes","Glutamic Acid","Glutamine","Rats","Rats, Wistar"],"mesh_terms":["Aging","Animals","Carbon Dioxide","Glutamine","Rats, Wistar","Glutamic Acid","Enterocytes","Rats"],"keywords":["Glutamine","Enterocyte","Glutamate receptor","Chemistry","Biochemistry","Internal medicine","Amino acid","Medicine","Small intestine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T11:28:09.930411Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}