{"doi":"10.1016/j.jneuroim.2014.04.010","title":"Effects of catecholamines on thymocyte apoptosis and proliferation depend on thymocyte microenvironment","abstract":null,"journal":"Journal of Neuroimmunology","year":2014,"id":652909,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1703433,"name":"Ana Rakin","orcid":null,"position":1,"is_corresponding":false},{"id":1703434,"name":"Ivan Pilipović","orcid":null,"position":2,"is_corresponding":false},{"id":1703435,"name":"Duško Kosec","orcid":null,"position":3,"is_corresponding":false},{"id":1703436,"name":"Jasmina Djikić","orcid":null,"position":4,"is_corresponding":false},{"id":1387518,"name":"Biljana Bufan","orcid":"0000-0003-1861-5700","position":5,"is_corresponding":false},{"id":1703437,"name":"Ivana Vujnović","orcid":null,"position":6,"is_corresponding":false},{"id":757394,"name":"Gordana Leposavić","orcid":"0000-0002-1341-7716","position":7,"is_corresponding":false},{"id":1703432,"name":"Katarina Radojević","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Effects of catecholamines on thymocyte apoptosis and proliferation depend on thymocyte microenvironment","abstract":"The present study, through quantification of tyrosine hydroxylase (TH) expression and catecholamine (CA) content in the presence and in the absence of α-methyl-p-tyrosine (AMPT), a TH inhibitor, in adult thymic organ (ATOC) and thymocyte culture, demonstrated that thymic cells produce CAs. In addition, in ATOC an increase in β2-adrenoceptor (AR) mRNA expression and β2-AR thymocyte surface density was registered. Furthermore, AMPT (10(-4)M), as propranolol (10(-4)M), augmented thymocyte apoptosis and diminished thymocyte proliferation in ATOC. Propranolol exerted these effects acting on CD3(high) thymocytes. However, in thymocyte cultures, propranolol (10(-6)M) acting on the same thymocyte subset exerted the opposing effect on thymocyte apoptosis and ConA-stimulated proliferation. This suggested that, depending on thymocyte microenvironment, differential effects can be induced through the same type of AR. Additionally, arterenol (10(-8) to 10(-6)M), similar to propranolol, diminished apoptosis, but increased ConA-stimulated thymocyte proliferation in thymocyte culture. However, differently from propranolol, arterenol affected manly CD3- thymocyte subset, which harbors majority of α1-AR+thymocytes. Additionally, arterenol showed a dose-dependent decrease in efficiency of thymocyte apoptosis and proliferation modulation with the rise in its concentration. Considering greater affinity of arterenol for α1-ARs than for β2-ARs, the previous findings could be attributable to increased engagement of β2-ARs with the rise of arterenol concentration. Consistently, in the presence of propranolol (10(-6)M), a β-AR blocker, the arterenol (10(-8)M) effects on thymocytes were augmented. In conclusion, thymic endogenous CAs, acting through distinct AR types and, possible, the same AR type (but in different cell microenvironment) may exert the opposing effects on thymocyte apoptosis/proliferation.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24837703","pmcid":null,"openalex_id":"https://openalex.org/W2095788784","authors":[],"funders":[{"funder_name":"Ministry of Education, Science and Technological Development of Republic of Serbia","grant_id":"175050","title":"Immune system plasticity during aging: Immunomodulatory capacity of oestrogens"}],"total_grants":1,"fwci":0.7799,"citation_percentile":0.70692879,"influential_citations":0,"citation_trend":[{"year":2015,"count":3},{"year":2016,"count":3},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2023,"count":3},{"year":2024,"count":1}],"oa_status":"closed","license":"Elsevier TDM","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0165572814001210?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0165572814001210?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jneuroim.2014.04.010","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24837703","host_type":"repository"},{"url":"http://farfar.pharmacy.bg.ac.rs/handle/123456789/2182","host_type":"repository"},{"url":"http://intor.torlakinstitut.com/handle/123456789/409","host_type":"repository"},{"url":"https://dx.doi.org/10.1016/j.jneuroim.2014.04.010","host_type":""},{"url":"https://intor.torlakinstitut.com/handle/123456789/409","host_type":""},{"url":"https://farfar.pharmacy.bg.ac.rs/handle/123456789/2182","host_type":""},{"url":"https://doi.org/https://doi.org/10.1016/j.jneuroim.2014.04.010","host_type":""}],"fields_of_study":["Receptor Mechanisms and Signaling","T-cell and B-cell Immunology","Protein Kinase Regulation and GTPase Signaling","0301 basic medicine","03 medical and health sciences","0302 clinical medicine","Adrenergic alpha-Agonists","Adrenergic beta-Antagonists","Animals","Apoptosis","Catecholamines","Cell Differentiation","Cell Proliferation","Cells, Cultured","Enzyme Inhibitors","Lymphocyte Activation","Norepinephrine","Organ Culture Techniques","Propranolol","Rats","Thymocytes","Thymus Gland","Tyrosine 3-Monooxygenase","alpha-Methyltyrosine"],"mesh_terms":["Adrenergic alpha-Agonists","Adrenergic beta-Antagonists","Animals","Catecholamines","Cell Differentiation","Cells, Cultured","Enzyme Inhibitors","Lymphocyte Activation","Norepinephrine","Organ Culture Techniques","Propranolol","Thymus Gland","Tyrosine 3-Monooxygenase","Apoptosis","alpha-Methyltyrosine","Cell Proliferation","Rats","Thymocytes"],"keywords":["Thymocyte","Propranolol","Apoptosis","Endocrinology","Internal medicine","Chemistry","Biology","T cell","Immunology","Biochemistry","Medicine","Immune system","Arterenol","Adult Thymus Organ Culture","Thymocyte Apoptosis","Thymocyte Culture","Thymocyte Proliferation","Tyrosine 3-Monooxygenase","Adrenergic beta-Antagonists","Thymus Gland","Lymphocyte Activation","Norepinephrine","Catecholamines","Organ Culture Techniques","Animals","Enzyme Inhibitors","Cells, Cultured","Cell Proliferation","Thymocytes","Cell Differentiation","Rats","alpha-Methyltyrosine","Adrenergic alpha-Agonists"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T17:26:46.086455Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}