{"doi":"10.1016/j.jneumeth.2016.01.025","title":"A flow cytometric approach to analyzing mature and progenitor endothelial cells following traumatic brain injury","abstract":null,"journal":"Journal of Neuroscience Methods","year":2016,"id":618532,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":814031,"name":"Oliver Umland","orcid":"0000-0002-8834-7804","position":1,"is_corresponding":false},{"id":1595741,"name":"Maria L. Cepero","orcid":null,"position":2,"is_corresponding":false},{"id":920818,"name":"Daniel J. Liebl","orcid":"0000-0003-2680-1220","position":3,"is_corresponding":false},{"id":1595740,"name":"Poincyane Assis-Nascimento","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A flow cytometric approach to analyzing mature and progenitor endothelial cells following traumatic brain injury","abstract":"<h4>Background</h4>Traumatic brain injury (TBI) continues to be a major source of death and disability worldwide, and one of the earliest and most profound deficits comes from vascular damage and breakdown of the blood-brain barrier (BBB). Cerebral vascular endothelial cells (cvECs) and endothelial progenitor cells (EPCs) have been shown to play essential roles in vessel repair and BBB stability, although their individual contributions remain poorly defined.<h4>New method</h4>We employ TruCount beads with flow cytometry to precisely quantify cvECs, EPCs, and peripheral leukocytes in the murine cortex after controlled cortical impact (CCI) injury.<h4>Results</h4>We found a significant reduction in the number of cvECs at 3 days post-injury (dpi), whereas the EPCs and invading peripheral leukocytes were significantly increased compared with sham controls. Proliferation studies demonstrate that both cvECs and EPCs are undergoing cell expansion in the first week post-injury. Furthermore, analysis of protein expression using mean fluorescence intensity found increases in PECAM-1, VEGFR-2, and VE-Cadherin expression per cell at 3 dpi, which is consistent with western blot analysis.<h4>Comparison with existing methods</h4>Classic methods of cell analysis, such as histological cell counts, in the traumatic injured brain are labor intensive, time-consuming, and potentially biased; whereas flow cytometry provides an efficient, non-biased approach to simultaneously quantify multiple cell types. However, conventional flow cytometry that employs capped events can provide misleading results in CNS injured tissues.<h4>Conclusions</h4>We demonstrate that TruCount quantification using flow cytometry is a powerful tool for quantifying mature and progenitor endothelial cell changes after TBI.","is_dataset_classified":null,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26854397","pmcid":"PMC4801778","openalex_id":"https://openalex.org/W2268084917","authors":[],"funders":[{"funder_name":"NINDS NIH HHS","grant_id":"P50 NS030291","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"R01 NS049545","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"F31 NS089325","title":null}],"total_grants":3,"fwci":0.9891,"citation_percentile":0.77510207,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":4},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4801778","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4801778","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S0165027016000509?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0165027016000509?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jneumeth.2016.01.025","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26854397","host_type":"repository"}],"fields_of_study":["Barrier Structure and Function Studies","Traumatic Brain Injury and Neurovascular Disturbances","Angiogenesis and VEGF in Cancer","Analysis of Variance","Animals","Antigens, CD","Brain Injuries, Traumatic","Cadherins","Cell Adhesion Molecules","Cell Proliferation","Disease Models, Animal","Endothelial Progenitor Cells","Flow Cytometry","Luminescent Proteins","Male","Mice","Mice, Transgenic","Phenylurea Compounds","Cadherin 5"],"mesh_terms":["Brain Injuries, Traumatic","Cadherin 5","Analysis of Variance","Animals","Disease Models, Animal","Flow Cytometry","Luminescent Proteins","Male","Mice, Transgenic","Phenylurea Compounds","Antigens, CD","Cell Adhesion Molecules","Cadherins","Cell Proliferation","Mice","Endothelial Progenitor Cells"],"keywords":["Flow cytometry","Progenitor cell","Endothelial progenitor cell","Western blot","Traumatic brain injury","Biology","Endothelial stem cell","Pathology","Cell","Cell biology","Immunology","Stem cell","Medicine","In vitro","Blood","endothelial progenitor cells","Endothelial Cells","Vessels"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Decent work and economic growth"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T04:38:58.740855Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}