{"doi":"10.1016/j.jneumeth.2014.01.005","title":"Avoiding the formation of vesicles by patch excision from Xenopus oocytes","abstract":null,"journal":"Journal of Neuroscience Methods","year":2014,"id":665557,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1737984,"name":"K. Benndorf","orcid":null,"position":1,"is_corresponding":false},{"id":1737983,"name":"S. Thon","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Avoiding the formation of vesicles by patch excision from Xenopus oocytes","abstract":"<h4>Background</h4>The patch-clamp technique is well-established to investigate the function of ion channels. Several patch configurations have been described, including the inside-out patch configuration providing the unique advantage of having free access to the patch from the cytosolic side. An inside-out patch is predominantly built from a cell-attached patch by pulling the patch pipette back. However, when using pipettes with high resistance (>8 MΩ), often a vesicle is formed instead of the desired inside-out patch, preventing proper recording of ion currents.<h4>New method</h4>Using quartz pipettes with high resistance we studied the benefit of a simple alternative excision manoeuvre that significantly enhances the efficiency to obtain an inside-out patch from Xenopus oocytes.<h4>Results</h4>We show that the formation of vesicles depends on the direction of patch excision: after a cell-attached patch has formed, pushing the patch pipette first into the depth of the oocyte and exposing the patch only then to the bath solution generated a success rate of 89% (16 out of 18) for a proper inside-out patch, as evaluated by the current flowing through HCN2 channels which were heterologously expressed in the oocytes.<h4>Comparison with existing method</h4>In contrast, with the same type of pipettes and oocytes only 22% (4 out of 18) of the patches developed HCN2 currents when pulling the pipette in the backward direction as usual.<h4>Conclusion</h4>The difference in the success rate favours the idea to use \"pushed inside-out patches\" instead of \"pulled inside-out patches\" when studying ion channels expressed in Xenopus oocytes.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24457054","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"Elsevier TDM","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0165027014000168?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0165027014000168?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jneumeth.2014.01.005","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/24457054","host_type":""},{"url":"https://dx.doi.org/10.1016/j.jneumeth.2014.01.005","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Oocytes","Animals","Xenopus laevis","Ion Channels","Artifacts","Patch-Clamp Techniques","Female"],"keywords":["Xenopus","vesicle","Inside-out Patch","Xenopus laevis","Patch-Clamp Techniques","Oocytes","Animals","Female","Artifacts","Ion Channels"],"sdg_mappings":[{"sdg_number":2,"sdg_label":"2. Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T11:29:37.306340Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}