{"doi":"10.1016/j.jneumeth.2008.09.024","title":"Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays","abstract":null,"journal":"Journal of Neuroscience Methods","year":2009,"id":605026,"datarank":1.519403896781292,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":1.0490797643919194,"self_endowment_contribution":0.47032413238937254,"citer_contribution":1.0490797643919194,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":18,"citers_with_citation_signal":18,"citers_with_endowment":18,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1552532,"name":"Leif Kongskov Larsen","orcid":null,"position":1,"is_corresponding":false},{"id":1552533,"name":"Jacob Jelsing","orcid":null,"position":2,"is_corresponding":false},{"id":1552534,"name":"Uwe Janßen","orcid":null,"position":3,"is_corresponding":false},{"id":1552535,"name":"Bernhard Gerstmayer","orcid":null,"position":4,"is_corresponding":false},{"id":200403,"name":"Niels Vrang","orcid":null,"position":5,"is_corresponding":false},{"id":1552531,"name":"Sarah Juel Paulsen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays","abstract":"In order to identify novel genes involved in appetite and body weight regulation we have developed a microarray based method suitable for detecting small changes in gene expression in discrete groups of hypothalamic neurons. The method is based on a combination of stereological sampling, laser capture microdissection (LCM), PCR based amplification (SuperAmp), and one-color cDNA microarray analysis. To validate the method we assessed and compared fasting induced changes in mRNA levels of Neuropeptide Y (NPY) and proopiomelanocortin (POMC) in the hypothalamic arcuate nucleus (ARC) of diet-induced obese rats using cDNA microarrays, quantitative PCR and in situ hybridization. All methods revealed statistically significant fasting-induced changes in NPY and POMC expression. An additional 3480 differentially expressed probes (fold change >1.22, t-test p=0.05) were identified in the microarray analysis. Our findings demonstrate a consistent gene expression pattern across three different gene expression detection methods and strongly suggest that LCM coupled microarray analysis combined with SuperAmp can be used as a semi-quantitative mRNA profiling tool. Importantly, the sensitivity of the method greatly improves the usefulness of the microarray technology for gene expression profiling in non-homogeneous tissues such as the brain.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18955080","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0165027008005773?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0165027008005773?httpAccept=text/plain","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Animals","Rats","Obesity","Pro-Opiomelanocortin","Dietary Fats","Neuropeptide Y","RNA, Messenger","Microdissection","Microarray Analysis","Fasting","Analysis of Variance","Gene Expression Profiling","Transcription, Genetic","Gene Expression Regulation","Male","Arcuate Nucleus of Hypothalamus"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T01:28:02.606746Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}