{"doi":"10.1016/j.jmr.2016.05.006","title":"A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T 1 relaxation enhancement","abstract":null,"journal":"Journal of Magnetic Resonance","year":2016,"id":667725,"datarank":0.611630616585858,"base_score":4.07753744390572,"endowment":4.07753744390572,"self_citation_contribution":0.611630616585858,"citation_network_contribution":0.0,"self_endowment_contribution":0.611630616585858,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":58,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":809950,"name":"Michael D. Bridges","orcid":"0000-0001-9999-9106","position":1,"is_corresponding":false},{"id":1743682,"name":"Carlos J. López","orcid":null,"position":2,"is_corresponding":false},{"id":1743683,"name":"Olga Yu. Rogozhnikova","orcid":null,"position":3,"is_corresponding":false},{"id":1743684,"name":"Dmitry V. Trukhin","orcid":null,"position":4,"is_corresponding":false},{"id":499993,"name":"Evan K. Brooks","orcid":null,"position":5,"is_corresponding":false},{"id":1069558,"name":"Victor M. Tormyshev","orcid":"0000-0003-4975-9993","position":6,"is_corresponding":false},{"id":411417,"name":"Howard J. Halpern","orcid":"0000-0001-7672-9269","position":7,"is_corresponding":false},{"id":317676,"name":"Wayne L. Hubbell","orcid":"0000-0003-0577-3312","position":8,"is_corresponding":false},{"id":834505,"name":"Zhongyu Yang","orcid":"0009-0002-2734-2046","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T 1 relaxation enhancement","abstract":"Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has become an important tool for measuring distances in proteins on the order of a few nm. For this purpose pairs of spin labels, most commonly nitroxides, are site-selectively introduced into the protein. Recent efforts to develop new spin labels are focused on tailoring the intrinsic properties of the label to either extend the upper limit of measurable distances at physiological temperature, or to provide a unique spectral lineshape so that selective pairwise distances can be measured in a protein or complex containing multiple spin label species. Triarylmethyl (TAM) radicals are the foundation for a new class of spin labels that promise to provide both capabilities. Here we report a new methanethiosulfonate derivative of a TAM radical that reacts rapidly and selectively with an engineered cysteine residue to generate a TAM containing side chain (TAM1) in high yield. With a TAM1 residue and Cu(2+) bound to an engineered Cu(2+) binding site, enhanced T1 relaxation of TAM should enable measurement of interspin distances up to 50Å at physiological temperature. To achieve favorable TAM1-labeled protein concentrations without aggregation, proteins are tethered to a solid support either site-selectively using an unnatural amino acid or via native lysine residues. The methodology is general and readily extendable to complex systems, including membrane proteins.","is_dataset_classified":null,"base_score":4.07753744390572,"endowment":4.07753744390572,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27214582","pmcid":"PMC4958593","openalex_id":"https://openalex.org/W2389594097","authors":[],"funders":[{"funder_name":"National Institute of Health","grant_id":"NIH/NEI EY005216","title":null},{"funder_name":"National Institute of Health","grant_id":"NIH/NEI EY00331","title":null},{"funder_name":"Russian Science Foundation","grant_id":"14-14-00922","title":null},{"funder_name":"Russian Foundation for Basic Research","grant_id":"14-03-93180","title":null},{"funder_name":"National Institute of Biomedical Imaging and Bioengineering","grant_id":"5P41EB002034","title":null},{"funder_name":"NEI NIH HHS","grant_id":"P30 EY000331","title":null},{"funder_name":"NEI NIH HHS","grant_id":"R01 EY005216","title":null},{"funder_name":"NEI NIH HHS","grant_id":"R37 EY005216","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA098575","title":null},{"funder_name":"NIBIB NIH HHS","grant_id":"P41 EB002034","title":null},{"funder_name":"Jules Stein Professorship Endowment","grant_id":"","title":null}],"total_grants":11,"fwci":11.2662,"citation_percentile":0.98100211,"influential_citations":0,"citation_trend":[{"year":2016,"count":6},{"year":2017,"count":7},{"year":2018,"count":6},{"year":2019,"count":7},{"year":2020,"count":5},{"year":2021,"count":8},{"year":2022,"count":7},{"year":2023,"count":9},{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"cc-by","oa_locations":[{"url":"https://escholarship.org/uc/item/6n95n6bg","host_type":"repository"},{"url":"https://escholarship.org/uc/item/6n95n6bg","host_type":"GREEN"},{"url":"https://escholarship.org/uc/item/6n95n6bg","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S1090780716300490?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1090780716300490?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jmr.2016.05.006","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27214582","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4958593","host_type":"repository"},{"url":"https://escholarship.org/uc/item/6h74p1nb","host_type":"repository"}],"fields_of_study":["Electron Spin Resonance Studies","Lanthanide and Transition Metal Complexes","Magnetism in coordination complexes","Chemistry","Medicine","Binding Sites","Electron Spin Resonance Spectroscopy","Proteins","Spin Labels","Temperature"],"mesh_terms":["Binding Sites","Electron Spin Resonance Spectroscopy","Proteins","Spin Labels","Temperature"],"keywords":["Site-directed spin labeling","Spin label","Electron paramagnetic resonance","Chemistry","Residue (chemistry)","Relaxation (psychology)","Cysteine","Nuclear magnetic resonance","Biochemistry","Spin Labeling","Saturation Recovery","Distance Measurement At Physiological Temperature","Relaxation Enhancement","Triarylmethyl Radical"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T18:47:56.931847Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}