{"doi":"10.1016/j.jmb.2017.02.004","title":"Optogenetic Control of Ca 2+ and Voltage-Dependent Large Conductance (BK) Potassium Channels","abstract":null,"journal":"Journal of Molecular Biology","year":2017,"id":645662,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1681314,"name":"Phillip G. Wood","orcid":null,"position":1,"is_corresponding":false},{"id":1681315,"name":"Ernst Bamberg","orcid":null,"position":2,"is_corresponding":false},{"id":1308841,"name":"Thomas Mager","orcid":"0000-0002-9571-7033","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Optogenetic Control of Ca 2+ and Voltage-Dependent Large Conductance (BK) Potassium Channels","abstract":"Ca 2 + concentration jumps for the activation of Ca 2 + -dependent ion channels or transporters can be obtained either by fast solution exchange or by the use of caged Ca 2 + . Here, we report on an alternate optogenetic method for the activation of Ca 2 + and voltage-dependent large conductance (BK) potassium channels. This was achieved through the use of the light-gated channelrhodopsin 2 variant, CatCh(Calcium translocating Channelrhodopsin) with enhanced Ca, which produces locally [Ca 2 + ] in the μM range on the inner side of the membrane, without significant [Ca 2 + ] increase in the cytosol. BK channel subunits α and β1 were expressed together with CatCh in HEK293 cells, and voltage and Ca 2 + dependence were analyzed. Light activation of endogenous BK channels under native conditions in astrocytes and glioma cells was also investigated. Additionally, BK channels were used as sensors for the calibration of the [Ca 2 + ] on the inner surface of the cell membrane.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28192090","pmcid":null,"openalex_id":"https://openalex.org/W2587684261","authors":[],"funders":[],"total_grants":0,"fwci":0.5461,"citation_percentile":0.55932011,"influential_citations":0,"citation_trend":[{"year":2017,"count":2},{"year":2018,"count":2},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":1}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S0022283617300682/pdf","host_type":"journal"},{"url":"https://www.sciencedirect.com/science/article/pii/S0022283617300682/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022283617300682?httpAccept=text/plain","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022283617300682?httpAccept=text/xml","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jmb.2017.02.004","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28192090","host_type":"repository"}],"fields_of_study":["Photoreceptor and optogenetics research","Ion channel regulation and function","Neuroscience and Neuropharmacology Research","Astrocytes","Cells, Cultured","Channelrhodopsins","Enzyme Activation","Fibroblasts","Gene Expression","Humans","Large-Conductance Calcium-Activated Potassium Channels","Neuroglia","Optogenetics"],"mesh_terms":["Channelrhodopsins","Astrocytes","Cells, Cultured","Enzyme Activation","Fibroblasts","Humans","Neuroglia","Gene Expression","Large-Conductance Calcium-Activated Potassium Channels","Optogenetics"],"keywords":["Optogenetics","BK channel","Potassium channel","Conductance","Potassium","Biophysics","Chemistry","Calcium-activated potassium channel","Inward-rectifier potassium ion channel","Nanotechnology","Biology","Materials science","Physics","Neuroscience","Ion channel","Biochemistry","Condensed matter physics","Channelrhodopsin","Minimal Invasive","Calcium Jump"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T09:44:09.228620Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}