{"doi":"10.1016/j.jmb.2009.05.080","title":"Solution Characterization of the Extracellular Region of CD147 and Its Interaction with Its Enzyme Ligand Cyclophilin A","abstract":null,"journal":"Journal of Molecular Biology","year":2009,"id":665735,"datarank":0.6476232170304466,"base_score":4.31748811353631,"endowment":4.31748811353631,"self_citation_contribution":0.6476232170304466,"citation_network_contribution":0.0,"self_endowment_contribution":0.6476232170304466,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":74,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":300021,"name":"Jasmina S. Redzic","orcid":null,"position":1,"is_corresponding":false},{"id":371918,"name":"Christopher C. Porter","orcid":"0000-0001-8774-0180","position":2,"is_corresponding":false},{"id":17897,"name":"Vyacheslav Yurchenko","orcid":"0000-0003-4765-3263","position":3,"is_corresponding":false},{"id":276950,"name":"Michael Bukrinsky","orcid":"0000-0002-8593-0770","position":4,"is_corresponding":false},{"id":1738432,"name":"Wladimir Labeikovsky","orcid":null,"position":5,"is_corresponding":false},{"id":1738433,"name":"Geoffrey S. Armstrong","orcid":null,"position":6,"is_corresponding":false},{"id":259682,"name":"Fengli Zhang","orcid":"0000-0003-4241-4503","position":7,"is_corresponding":false},{"id":1738434,"name":"Nancy G. Isern","orcid":null,"position":8,"is_corresponding":false},{"id":232041,"name":"James DeGregori","orcid":"0000-0002-1287-1976","position":9,"is_corresponding":false},{"id":1738435,"name":"Robert Hodges","orcid":null,"position":10,"is_corresponding":false},{"id":1738436,"name":"Elan Zohar Eisenmesser","orcid":null,"position":11,"is_corresponding":false},{"id":541674,"name":"Jennifer Schlegel","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Solution Characterization of the Extracellular Region of CD147 and Its Interaction with Its Enzyme Ligand Cyclophilin A","abstract":"The CD147 receptor plays an integral role in numerous diseases by stimulating the expression of several protein families and serving as the receptor for extracellular cyclophilins; however, neither CD147 nor its interactions with its cyclophilin ligands have been well characterized in solution. CD147 is a unique protein in that it can function both at the cell membrane and after being released from cells where it continues to retain activity. Thus, the CD147 receptor functions through at least two mechanisms that include both cyclophilin-independent and cyclophilin-dependent modes of action. In regard to CD147 cyclophilin-independent activity, CD147 homophilic interactions are thought to underlie its activity. In regard to CD147 cyclophilin-dependent activity, cyclophilin/CD147 interactions may represent a novel means of signaling since cyclophilins are also peptidyl-prolyl isomerases. However, direct evidence of catalysis has not been shown within the cyclophilin/CD147 complex. In this report, we have characterized the solution behavior of the two most prevalent CD147 extracellular isoforms through biochemical methods that include gel-filtration and native gel analysis as well as directly through multiple NMR methods. All methods indicate that the extracellular immunoglobulin-like domains are monomeric in solution and, thus, suggest that CD147 homophilic interactions in vivo are mediated through other partners. Additionally, using multiple NMR techniques, we have identified and characterized the cyclophilin target site on CD147 and have shown for the first time that CD147 is also a substrate of its primary cyclophilin enzyme ligand, cyclophilin A.","is_dataset_classified":null,"base_score":4.31748811353631,"endowment":4.31748811353631,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19500591","pmcid":"PMC2940942","openalex_id":"https://openalex.org/W2077426548","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R56 AI081152","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM68928","title":null},{"funder_name":"NCI NIH HHS","grant_id":"T32 CA082086","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI081152","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA109657","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI060720","title":null},{"funder_name":"National Science Foundation","grant_id":"0820567","title":"Probing Dynamics Within an Enzyme Family"},{"funder_name":"National Science Foundation","grant_id":"0654118","title":"National High Magnetic Field Laboratory Renewal"}],"total_grants":8,"fwci":2.2677,"citation_percentile":0.88027682,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":2},{"year":2014,"count":8},{"year":2015,"count":6},{"year":2016,"count":4},{"year":2017,"count":5},{"year":2018,"count":3},{"year":2019,"count":4},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":7},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":5}],"oa_status":"green","license":"Elsevier TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2940942","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2940942","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S0022283609006743?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022283609006743?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jmb.2009.05.080","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19500591","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2940942","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2940942?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1016/j.jmb.2009.05.080","host_type":""},{"url":"https://dx.doi.org/10.1016/j.jmb.2009.05.080","host_type":""}],"fields_of_study":["Signaling Pathways in Disease","Toxin Mechanisms and Immunotoxins","0301 basic medicine","0303 health sciences","03 medical and health sciences","Basigin","Catalysis","Cyclophilin A","Humans","Models, Molecular","Protein Binding","Protein Folding","Protein Isoforms","Protein Structure, Secondary","Protein Structure, Tertiary","Recombinant Proteins","Syndecan-1"],"mesh_terms":["Catalysis","Humans","Models, Molecular","Protein Binding","Recombinant Proteins","Protein Structure, Secondary","Protein Structure, Tertiary","Protein Folding","Protein Isoforms","Cyclophilin A","Basigin","Syndecan-1"],"keywords":["Cyclophilin","Extracellular","Chemistry","Enzyme","Characterization (materials science)","Cyclophilin A","Ligand (biochemistry)","Biochemistry","Biophysics","Crystallography","Biology","Molecular biology","Materials science","Nanotechnology","Receptor","Models, Molecular","Protein Folding","Article","Catalysis","Protein Structure, Secondary","Recombinant Proteins","Protein Structure, Tertiary","Basigin","Humans","Protein Isoforms","Syndecan-1","Protein Binding"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T12:07:12.186259Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}