{"doi":"10.1016/j.jmb.2007.06.071","title":"Differential Requirement of Unfolded Protein Response Pathway for Calreticulin Expression in Caenorhabditis elegans","abstract":null,"journal":"Journal of Molecular Biology","year":2007,"id":662331,"datarank":0.515098080672772,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.0,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":817605,"name":"Gunasekaran Singaravelu","orcid":null,"position":1,"is_corresponding":false},{"id":1729041,"name":"Byung-Jae Park","orcid":null,"position":2,"is_corresponding":false},{"id":721372,"name":"Joohong Ahnn","orcid":null,"position":3,"is_corresponding":false},{"id":1729040,"name":"Dukgyu Lee","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Differential Requirement of Unfolded Protein Response Pathway for Calreticulin Expression in Caenorhabditis elegans","abstract":"Accumulation of unfolded proteins in the endoplasmic reticulum triggers the unfolded protein response (UPR) pathway, which increases the expression of chaperones to maintain the homeostasis. Calreticulin is a calcium-binding chaperone located in the lumen of endoplasmic reticulum (ER). Here we show that in response to a UPR inducing reagent, tunicamycin, the expression of calreticulin (crt-1) is specifically up-regulated in Caenorhabditis elegans. Tunicamycin (TM) induced expression of the crt-1 requires IRE-1 and XBP-1 but is ATF-6 and PEK-1 independent. Analysis of the crt-1 promoter reveals a putative XBP-1 binding site at the -284 to -278 bp region, which was shown to be necessary for TM-mediated induction. Genetic analysis of crt-1 mutants and mutants of UPR pathway genes show various degrees of developmental arrest upon TM treatment. Our results suggest that the TM-induced UPR pathway culminates in the up-regulation of crt-1, which protects the worm from deleterious accumulation of unfolded proteins in the ER. Knockdown of the crt-1, pdi-2, or pdi-3 increased the crt-1 expression, whereas knockdown of the hsp-3 or hsp-4 did not have any effect on crt-1 expression, indicating the existence of complex compensatory networks to cope up with ER stress.","is_dataset_classified":null,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17651753","pmcid":null,"openalex_id":"https://openalex.org/W1970482691","authors":[],"funders":[],"total_grants":0,"fwci":0.4858,"citation_percentile":0.60172974,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":1},{"year":2014,"count":4},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0022283607008807?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022283607008807?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jmb.2007.06.071","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17651753","host_type":"repository"}],"fields_of_study":["Endoplasmic Reticulum Stress and Disease","Genetics, Aging, and Longevity in Model Organisms","Animals","Caenorhabditis elegans","Calreticulin","Endoplasmic Reticulum","Feedback, Physiological","Gene Expression Regulation","Heat-Shock Proteins","Mutation","Protein Denaturation","Protein Disulfide-Isomerases","Protein Folding","Response Elements","Tunicamycin","Up-Regulation"],"mesh_terms":["Animals","Endoplasmic Reticulum","Gene Expression Regulation","Heat-Shock Proteins","Mutation","Protein Denaturation","Tunicamycin","Up-Regulation","Caenorhabditis elegans","Protein Folding","Protein Disulfide-Isomerases","Response Elements","Feedback, Physiological","Calreticulin"],"keywords":["Calreticulin","Unfolded protein response","Endoplasmic reticulum","Tunicamycin","Cell biology","Gene knockdown","Caenorhabditis elegans","ATF6","Biology","Calnexin","Mutant","Chaperone (clinical)","KDEL","Gene","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T13:55:33.465768Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}